Journal Published Online: 01 March 1994
Volume 39, Issue 2

Prenatal Exclusion of Paternity by PCR-FLP Analysis of VNTR

CODEN: JFSCAS

Abstract

A prenatal paternity test was requested by a 30-year-old woman. Variable number of tandem repeat (VNTR) systems were used for DNA analysis by means of amplification and electrophoresis followed by ethidium bromide staining or Southern blotting and oligonucleotide hybridization. Exclusion of paternity could be established on the basis of the great polymorphism and heterozygosity indexes of these genetic systems. This rapid method presents several advantages in contrast with other recombinant DNA techniques such as HLA class II oligotyping or RFLP.

Author Information

Arroyo, E
Laboratorio de Histocompatibilidad, Centro de Transfusión, de la Comunidad de Madrid, Madrid, Spain Sección de Biología Forense, Dpto. de Toxicología y Legislación Sanitaria, Facultad de Medicina, Pabellón VII, Universidad Complutense, Madrid, Spain
García-Sánchez, F
Laboratorio de Histocompatibilidad, Centro de Transfusión, de la Comunidad de Madrid, Madrid, Spain
Gómez-Reino, F
Laboratorio de Histocompatibilidad, Centro de Transfusión, de la Comunidad de Madrid, Madrid, Spain
Ruiz de la Cuesta, JM
Sección de Biología Forense, Dpto. de Toxicología y Legislación Sanitaria, Facultad de Medicina, Pabellón VII, Universidad Complutense, Madrid, Spain
Vicario, JL
Laboratorio de Histocompatibilidad, Centro de Transfusión, de la Comunidad de Madrid, Madrid, Spain
Pages: 7
Price: $25.00
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Stock #: JFS13631J
ISSN: 0022-1198
DOI: 10.1520/JFS13631J