ISSN: 0022-1198
CODEN: JFSCA
Page Count: 7

Prenatal Exclusion of Paternity by PCR-FLP Analysis of VNTR
Arroyo, E

Gómez-Reino, F
Laboratorio de Histocompatibilidad, Centro de Transfusión, de la Comunidad de Madrid,

García-Sánchez, F
Laboratorio de Histocompatibilidad, Centro de Transfusión, de la Comunidad de Madrid,

Vicario, JL
Laboratorio de Histocompatibilidad, Centro de Transfusión, de la Comunidad de Madrid,

Ruiz de la Cuesta, JM
Sección de Biología Forense, Dpto. de Toxicología y Legislación Sanitaria, Facultad de Medicina, Pabellón VII, Universidad Complutense,

Abstract

A prenatal paternity test was requested by a 30-year-old woman. Variable number of tandem repeat (VNTR) systems were used for DNA analysis by means of amplification and electrophoresis followed by ethidium bromide staining or Southern blotting and oligonucleotide hybridization. Exclusion of paternity could be established on the basis of the great polymorphism and heterozygosity indexes of these genetic systems. This rapid method presents several advantages in contrast with other recombinant DNA techniques such as HLA class II oligotyping or RFLP.

Keywords:
pathology and biology, paternity testing, VNTR, prenatal diagnosis, DNA amplification

Paper ID: JFS13631J
DOI: 10.1520/JFS13631J
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