ISSN: 0022-1198
CODEN: JFSCA
Page Count: 5

GC/MS Quantitation of Benzoylecgonine Following Liquid-Liquid Extraction of Urine
Gerlits, J
Criminalist, Utah State Crime Lab, Southern Utah University, UT

(Received 5 June 1992; accepted 12 February 1993)

Abstract

An analytical procedure for the determination of benzoylecgonine in urine by GC/MS using a two step liquid-liquid extraction scheme has been developed. Benzoylecgonine is one of the principle metabolites of cocaine. The extraction uses a wash step prior to extraction and derivatization and requires 0.5 mL of urine. Benzoylecgonine is extracted in good % recovery at a basic pH by using 4 mL of dichloromethane. The method is accurate and sensitive and allows the quantitation of benzoylecgonine in urine at or below the National Institute on Drug Abuse confirmation cutoff level of 150 ng/mL.

Keywords:
toxicology, cocaine, metabolite, derivatization

Paper ID: JFS13525J
DOI: 10.1520/JFS13525J
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