ISSN: 0022-1198
CODEN: JFSCA
Page Count: 7

Identification of Human DNA in Complex Biological Samples Using the Alu Polymerase Chain Reaction
Kaufman, DG
Postdoctoral Research Fellows, Research Technician, Associate Professor, and Professor, University of North Carolina, N.C.

Smith, GJ
Postdoctoral Research Fellows, Research Technician, Associate Professor, and Professor, University of North Carolina, N.C.

Tsongalis, GJ
Postdoctoral Research Fellows, Research Technician, Associate Professor, and Professor, University of North Carolina, N.C.

Esch, GL
Postdoctoral Research Fellows, Research Technician, Associate Professor, and Professor, University of North Carolina, N.C.

Coleman, WB
Postdoctoral Research Fellows, Research Technician, Associate Professor, and Professor, University of North Carolina, N.C.

(Received 12 August 1992; accepted 20 January 1993)

Abstract

Alu-Polymerase chain reaction (PCR) was used to amplify human DNA from complex mixed sources of DNA. Amplification of human DNA sequences by Alu-PCR could be accomplished in samples containing low concentrations of template in the presence of excess heterologous DNA sequences. Thus, sensitivity and specificity are maintained in complex DNA mixtures allowing positive identification of the presence of human DNA sequences by this technique.

Keywords:
forensic science, Alu polymerase chain reaction (Alu-PCR), human identification, DNA

Paper ID: JFS13495J
DOI: 10.1520/JFS13495J
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