Identification of Human DNA in Complex Biological Samples Using the Alu Polymerase Chain Reaction

    Volume 38, Issue 4 (July 1993)

    ISSN: 0022-1198

    CODEN: JFSOAD

    Page Count: 7


    Kaufman, DG
    Postdoctoral Research Fellows, Research Technician, Associate Professor, and Professor, University of North Carolina, Chapel Hill, N.C.

    Smith, GJ
    Postdoctoral Research Fellows, Research Technician, Associate Professor, and Professor, University of North Carolina, Chapel Hill, N.C.

    Tsongalis, GJ
    Postdoctoral Research Fellows, Research Technician, Associate Professor, and Professor, University of North Carolina, Chapel Hill, N.C.

    Esch, GL
    Postdoctoral Research Fellows, Research Technician, Associate Professor, and Professor, University of North Carolina, Chapel Hill, N.C.

    Coleman, WB
    Postdoctoral Research Fellows, Research Technician, Associate Professor, and Professor, University of North Carolina, Chapel Hill, N.C.

    (Received 12 August 1992; accepted 20 January 1993)

    Abstract

    Alu-Polymerase chain reaction (PCR) was used to amplify human DNA from complex mixed sources of DNA. Amplification of human DNA sequences by Alu-PCR could be accomplished in samples containing low concentrations of template in the presence of excess heterologous DNA sequences. Thus, sensitivity and specificity are maintained in complex DNA mixtures allowing positive identification of the presence of human DNA sequences by this technique.


    Paper ID: JFS13495J

    DOI: 10.1520/JFS13495J

    ASTM International
    is a member of CrossRef.

    Author
    Title Identification of Human DNA in Complex Biological Samples Using the Alu Polymerase Chain Reaction
    Symposium , 0000-00-00
    Committee E30