Purification of Forensic Specimens for the Polymerase Chain Reaction (PCR) Analysis

    Volume 38, Issue 3 (May 1993)

    ISSN: 0022-1198

    CODEN: JFSOAD

    Page Count: 11


    Akane, A
    Forensic Scientist, professor and associate professor, Shimane Medical University, Izumo,

    Nakamura, H
    Research scientists, Criminal Investigation Laboratory, Shimane Prefectural Police Headquarters, Matsue,

    Shiono, H
    Forensic Scientist, professor and associate professor, Shimane Medical University, Izumo,

    Matsubara, K
    Forensic Scientist, professor and associate professor, Shimane Medical University, Izumo,

    Hasegawa, M
    Research scientists, Criminal Investigation Laboratory, Shimane Prefectural Police Headquarters, Matsue,

    Kagawa, M
    Research scientists, Criminal Investigation Laboratory, Shimane Prefectural Police Headquarters, Matsue,

    (Received 27 June 1992; accepted 28 August 1992)

    Abstract

    Purification methods of deoxyribonucleic acid (DNA) from degraded and contaminated forensic samples were investigated for polymerase chain reaction (PCR) analysis. DNA extracted from putrefied tissue or bloodstains sometimes contained the copurified contaminant, that was identified as the porphyrin compound (hematin). When contaminated but less degraded DNA was analyzed by PCR, it was necessary to eliminate the impurity by anion exchange column chromatography or chelating resin preparation, and ultrafiltration using Centricon microconcentrators. When highly degraded DNA was analyzed, trace amounts of high molecular weight DNA was recovered by electroelution method, and then further purified by both column chromatography and ultrafiltration. From thus purified samples, the amelogenin gene for sex determination could be amplified by dual PCR technique.


    Paper ID: JFS13457J

    DOI: 10.1520/JFS13457J

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    Title Purification of Forensic Specimens for the Polymerase Chain Reaction (PCR) Analysis
    Symposium , 0000-00-00
    Committee E30