Mitochondrial DNA Sequence Analysis of Human Skeletal Remains: Identification of Remains from the Vietnam War

    Volume 38, Issue 3 (May 1993)

    ISSN: 0022-1198

    CODEN: JFSOAD

    Page Count: 12


    Merril, CR
    Postdoctoral Fellow and Chief, The Laboratory of Biochemical Genetics, The National Institutes of Mental Health, Neuroscience Center, Washington, DC

    Fisher, DL
    Research Scientist, DNA Analyst, Administrator, and Chief, The Armed Forces DNA Identification Laboratory. The Armed Forces Institute of Pathology, Washington, DC

    Canik, JJ

    Mitchell, LG
    Postdoctoral Fellow and Chief, The Laboratory of Biochemical Genetics, The National Institutes of Mental Health, Neuroscience Center, Washington, DC

    Holland, MM
    Research Scientist, DNA Analyst, Administrator, and Chief, The Armed Forces DNA Identification Laboratory. The Armed Forces Institute of Pathology, Washington, DC

    Weedn, VW
    Research Scientist, DNA Analyst, Administrator, and Chief, The Armed Forces DNA Identification Laboratory. The Armed Forces Institute of Pathology, Washington, DC

    Rodriquez, WC
    Forensic Anthropologist, The Office of the Armed Forces Medical Examiner, The Armed Forces Institute of Pathology, Washington, DC

    (Received 4 May 1992; accepted 17 November 1992)

    Abstract

    Deoxyribonucleic acid (DNA) sequence analysis of the control region of the mitochondrial DNA (mtDNA) genome was used to identify human skeletal remains returned to the United States government by the Vietnamese government in 1984. The postmortem interval was thought to be 24 years at the time of testing, and the remains presumed to be an American service member. DNA typing methods using nuclear genomic DNA, HLA-DQ alpha [1] and the variable number of tandem repeat (VNTR) locus D1S80 [2], were unsuccessful using the polymerase chain reaction (PCR) [3]. Amplification of a portion of the mtDNA control region was performed, and the resulting PCR product subjected to DNA sequence analysis. The DNA sequence generated from the skeletal remains was identical to the maternal reference sequence, as well as the sequence generated from two siblings (sisters). The sequence was unique when compared to more than 650 DNA sequences found both in the literature and provided by personal communications. The individual sequence polymorphisms were present in only 23 of the more than 1300 nucleotide positions analyzed. These results support the observation [4] that in cases where conventional DNA typing is unavailable, mtDNA sequencing can be used for human remains identification.


    Paper ID: JFS13439J

    DOI: 10.1520/JFS13439J

    ASTM International
    is a member of CrossRef.

    Author
    Title Mitochondrial DNA Sequence Analysis of Human Skeletal Remains: Identification of Remains from the Vietnam War
    Symposium , 0000-00-00
    Committee E30