Journal Published Online: 01 March 1993
Volume 38, Issue 2

A Rapid Dot-Blot Method for Species Identification of Bloodstains

CODEN: JFSCAS

Abstract

A very simple and rapid test for species identification is reported. Extracts of bloodstains were applied to a synthetic porous membrane and dried. The membrane was then quenched with glycine buffered saline containing BSA and Tween 20. A suspension of colloidal gold particles (GP) coated with rabbit antiserum to human IgG was poured onto, gently whirled and aspirated through the membrane. Spots from the human and monkey bloodstains became red, whereas those from other species of animals remained unstained. This test was completed within 3 to 4 min, and the antibody-coated GP reagent was prepared within 20 min using a very small quantity of antiserum. Cellulose acetate membranes of 0.45 μm or more in pore size were appropriate to this test.

Author Information

Matsuzawa, S
Juntendo University School of Medicine, Tokyo, Japan
Kimura, H
Juntendo University School of Medicine, Tokyo, Japan
Itoh, Y
Juntendo University School of Medicine, Tokyo, Japan
Wang, H
Juntendo University School of Medicine, Tokyo, Japan
Nakagawa, T
Faculty of Medicine, University of Tokyo, Tokyo, Japan
Pages: 7
Price: $25.00
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Stock #: JFS13427J
ISSN: 0022-1198
DOI: 10.1520/JFS13427J