Volume 38, Issue 2 (March 1993)

    A Rapid Dot-Blot Method for Species Identification of Bloodstains

    (Received 9 December 1991; accepted 16 July 1992)

    CODEN: JFSOAD

      Format Pages Price  
    PDF 7 $25   ADD TO CART


    Abstract

    A very simple and rapid test for species identification is reported. Extracts of bloodstains were applied to a synthetic porous membrane and dried. The membrane was then quenched with glycine buffered saline containing BSA and Tween 20. A suspension of colloidal gold particles (GP) coated with rabbit antiserum to human IgG was poured onto, gently whirled and aspirated through the membrane. Spots from the human and monkey bloodstains became red, whereas those from other species of animals remained unstained. This test was completed within 3 to 4 min, and the antibody-coated GP reagent was prepared within 20 min using a very small quantity of antiserum. Cellulose acetate membranes of 0.45 μm or more in pore size were appropriate to this test.


    Author Information:

    Kimura, H
    Professor, Docents, and Research Fellow, Juntendo University School of Medicine, Tokyo,

    Wang, H
    Professor, Docents, and Research Fellow, Juntendo University School of Medicine, Tokyo,

    Matsuzawa, S
    Professor, Docents, and Research Fellow, Juntendo University School of Medicine, Tokyo,

    Nakagawa, T
    Staff Member, Faculty of Medicine, University of Tokyo, Tokyo,

    Itoh, Y
    Professor, Docents, and Research Fellow, Juntendo University School of Medicine, Tokyo,


    Stock #: JFS13427J

    ISSN: 0022-1198

    DOI: 10.1520/JFS13427J

    ASTM International
    is a member of CrossRef.

    Author
    Title A Rapid Dot-Blot Method for Species Identification of Bloodstains
    Symposium , 0000-00-00
    Committee E30