A Rapid Dot-Blot Method for Species Identification of Bloodstains

    Volume 38, Issue 2 (March 1993)

    ISSN: 0022-1198

    CODEN: JFSOAD

    Page Count: 7


    Kimura, H
    Professor, Docents, and Research Fellow, Juntendo University School of Medicine, Tokyo,

    Wang, H
    Professor, Docents, and Research Fellow, Juntendo University School of Medicine, Tokyo,

    Matsuzawa, S
    Professor, Docents, and Research Fellow, Juntendo University School of Medicine, Tokyo,

    Nakagawa, T
    Staff Member, Faculty of Medicine, University of Tokyo, Tokyo,

    Itoh, Y
    Professor, Docents, and Research Fellow, Juntendo University School of Medicine, Tokyo,

    (Received 9 December 1991; accepted 16 July 1992)

    Abstract

    A very simple and rapid test for species identification is reported. Extracts of bloodstains were applied to a synthetic porous membrane and dried. The membrane was then quenched with glycine buffered saline containing BSA and Tween 20. A suspension of colloidal gold particles (GP) coated with rabbit antiserum to human IgG was poured onto, gently whirled and aspirated through the membrane. Spots from the human and monkey bloodstains became red, whereas those from other species of animals remained unstained. This test was completed within 3 to 4 min, and the antibody-coated GP reagent was prepared within 20 min using a very small quantity of antiserum. Cellulose acetate membranes of 0.45 μm or more in pore size were appropriate to this test.


    Paper ID: JFS13427J

    DOI: 10.1520/JFS13427J

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    Title A Rapid Dot-Blot Method for Species Identification of Bloodstains
    Symposium , 0000-00-00
    Committee E30