Detection of Prostate Specific Antigen by ELISA

    Volume 38, Issue 2 (March 1993)

    ISSN: 0022-1198

    CODEN: JFSOAD

    Page Count: 9


    Johnson, ED
    Forensic Chemists, US Army Crime Lab, USACIL-CONUS, Fort Gillem, GA

    Kotowski, TM
    Forensic Chemists, US Army Crime Lab, USACIL-CONUS, Fort Gillem, GA

    (Received 29 January 1992; accepted 4 August 1992)

    Abstract

    A method for the detection of prostate specific antigen (PSA or P30) by enzyme linked immunosorbent assay (ELISA) has been developed. The method was evaluated for sensitivity and specificity and compared to other methods of semen identification on numerous casework samples. A sensitivity of less than 1 ng/mL PSA is routinely achieved. Numerous domestic contaminants and body fluids were tested and none were found to give a false-positive result. However, false-negative results did occur in samples contaminated with detergents. Additionally, some poorly preserved items of evidence did not give positive results where expected. In spite of these minor limitations, this method was found to be highly sensitive and specific and an efficient method for identifying semen in forensic casework.


    Paper ID: JFS13403J

    DOI: 10.1520/JFS13403J

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    Author
    Title Detection of Prostate Specific Antigen by ELISA
    Symposium , 0000-00-00
    Committee E30