Volume 38, Issue 1 (January 1993)

    Extraction, Evaluation, and Amplification of DNA from Decalcified and Undecalcified United States Civil War Bone

    (Received 5 March 1992; accepted 11 May 1992)

    CODEN: JFSOAD

      Format Pages Price  
    PDF 9 $25   ADD TO CART


    Abstract

    Deoxyribonucleic acid (DNA) was extracted from documented skeletal specimens of U.S. Civil War soldiers to determine the need for decalcification prior to extraction. The polymerase chain reaction (PCR) was performed to determine if the calcification state had an effect on the ability to amplify the extracts and to determine how successful amplification would be with these aged specimens. Bone samples were pulverized to a fine powder and divided into two sets. One set of samples was decalcified and the other set left undecalcified. Both sets were extracted using an organic procedure. The results demonstrate that decalcification is not a necessary step in the extraction process and that the yield of DNA is generally two times greater when decalcification is omitted. Furthermore, the calcification state had no effect on the ability to perform the PCR. Although the extracted DNA was very degraded, a 410 base pair (bp) segment of the mitochondrial DNA (mtDNA) control region was amplified. These results suggest that DNA can be extracted and amplified from 125 year old bone without decalcification, which may assist in the identity of modern and historic forensic specimens.


    Author Information:

    Wilcox, AW
    Curator and Anatomical Collections Manager, National Museum of Health and Medicine, Armed Forces Institute of Pathology, Washington, DC

    Fisher, DL
    DNA Analyst, Research Scientist, and Chief, Armed Forces DNA Identification Laboratory, Office of the Armed Forces Medical Examiner, Armed Forces Institute of Pathology, Washington, D.C.

    Mitchell, L
    Research Scientist, Institute of Mental Health, Neuroscience Center, National Institutes of Health, Washington, DC

    Holland, MM
    DNA Analyst, Research Scientist, and Chief, Armed Forces DNA Identification Laboratory, Office of the Armed Forces Medical Examiner, Armed Forces Institute of Pathology, Washington, D.C.

    Wadhams, M
    Research Associate, Cellmark Diagnostics, Germantown, MD

    Sledzik, PS
    Curator and Anatomical Collections Manager, National Museum of Health and Medicine, Armed Forces Institute of Pathology, Washington, DC

    Weedn, VW
    DNA Analyst, Research Scientist, and Chief, Armed Forces DNA Identification Laboratory, Office of the Armed Forces Medical Examiner, Armed Forces Institute of Pathology, Washington, D.C.


    Stock #: JFS13376J

    ISSN: 0022-1198

    DOI: 10.1520/JFS13376J

    ASTM International
    is a member of CrossRef.

    Author
    Title Extraction, Evaluation, and Amplification of DNA from Decalcified and Undecalcified United States Civil War Bone
    Symposium , 0000-00-00
    Committee E30