ISSN: 0022-1198
CODEN: JFSCA
Page Count: 4

The Application of Immunoblotting to the Phenotyping of Haptoglobin
Thomas, AS
Forensic Scientists, Forensic Biology section, State Health Laboratory, Queensland

Cox, KJ
Forensic Scientists, Forensic Biology section, State Health Laboratory, Queensland

(Received 6 January 1992; accepted 14 February 1992)

Abstract

An immunoblotting method for phenotyping haptoglobin in serum and blood-stains has been developed. Haptoglobin isoproteins were separated by polyacrylamide gradient gel electrophoresis and then transferred to nitrocellulose by electroblotting. The use of 1 mm gels facilitated more rapid and effective transfer than conventional 3 mm thick gels. Nitrocellulose blots were developed by double antibody enzyme immunoassay. The detection limit for serum and bloodstains was improved 16 times compared to conventional staining using O-tolidine. The method could detect haptoglobin phenotypes from 0.001 μl of whole blood. This detection limit is approximately 8 times lower than that of group specific-component analysis by immunoblotting.

Keywords:
pathology and biology, immunoblotting, phenotyping, haptoglobin

Paper ID: JFS13355J
DOI: 10.1520/JFS13355J
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