The Application of Immunoblotting to the Phenotyping of Haptoglobin

    Volume 37, Issue 6 (November 1992)

    ISSN: 0022-1198

    CODEN: JFSOAD

    Page Count: 4


    Thomas, AS
    Forensic Scientists, Forensic Biology section, State Health Laboratory, Brisbane, Queensland

    Cox, KJ
    Forensic Scientists, Forensic Biology section, State Health Laboratory, Brisbane, Queensland

    (Received 6 January 1992; accepted 14 February 1992)

    Abstract

    An immunoblotting method for phenotyping haptoglobin in serum and blood-stains has been developed. Haptoglobin isoproteins were separated by polyacrylamide gradient gel electrophoresis and then transferred to nitrocellulose by electroblotting. The use of 1 mm gels facilitated more rapid and effective transfer than conventional 3 mm thick gels. Nitrocellulose blots were developed by double antibody enzyme immunoassay. The detection limit for serum and bloodstains was improved 16 times compared to conventional staining using O-tolidine. The method could detect haptoglobin phenotypes from 0.001 μl of whole blood. This detection limit is approximately 8 times lower than that of group specific-component analysis by immunoblotting.


    Paper ID: JFS13355J

    DOI: 10.1520/JFS13355J

    ASTM International
    is a member of CrossRef.

    Author
    Title The Application of Immunoblotting to the Phenotyping of Haptoglobin
    Symposium , 0000-00-00
    Committee E30