ISSN: 0022-1198
CODEN: JFSCA
Page Count: 12

Robotic Method for the Analysis of Morphine and Codeine in Urine
Vidal, DL
Senior Criminalist, Los Angeles County Sheriff's Department, Scientific Services Bureau, Toxicology Section, CA

Ting, EJ
Senior Criminalist, Los Angeles County Sheriff's Department, Scientific Services Bureau, Toxicology Section, CA

Taylor, RW
Senior Criminalist, Los Angeles County Sheriff's Department, Scientific Services Bureau, Toxicology Section, CA

Le, SD
Supervising Criminalist, Los Angeles County Sheriff's Department, Scientific Services Bureau, Toxicology Section,

Perez, SL
Criminalistics Laboratory Technician, Los Angeles County Sheriff's Department, Scientific Services Bureau, Toxicology Section,

(Received 18 November 1991; accepted 30 December 1991)

Abstract

A totally automated procedure has been developed for the detection and quantitation of morphine and codeine in urine case samples. The samples were initially screened for these drugs by a Syva^R EMIT Toxicology System (ETS™). A Zymate™ laboratory robotic system confirms positive samples from Syva^R ETS by performing the hydrolysis, extraction, and derivatization of morphine and codeine. The derivatized morphine and codeine were detected using gas chromatography/mass spectrometry (GC/MS). Enzymatic hydrolysis conditions were experimentally optimized during method development. The automation of these procedures has proven to be reliable and efficient.

Keywords:
toxicology, robotics, codeine, morphine, enzymatic hydrolysis

Paper ID: JFS13316J
DOI: 10.1520/JFS13316J
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