Volume 37, Issue 4 (July 1992)

    Simultaneous Quantitation of Delta-9-tetrahydrocannabinol (THC) and 11-Nor-9-carboxy-delta-9-tetrahydrocannabinol (THC-COOH) in Serum by GC/MS Using Deuterated Internal Standards and Its Application to a Smoking Study and Forensic Cases

    (Received 16 October 1991; accepted 18 November 1991)

    CODEN: JFSOAD

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    Abstract

    A new procedure for the simultaneous detection of delta-9-tetrahydrocannabinol (THC) and its major metabolite, 11-nor-9-carboxy-delta-9-tetrahydrocannabinol (THC-COOH) in serum has been evaluated. The method combines rapid, efficient, solid-phase extraction and simple derivatization by methylation. Analysis and quantitation is performed by gas chromatography/mass spectrometry (GC/MS) using deuterated cannabinoids as internal standards (IS). Reproducibility and sensitivity of the method are good.

    The procedure is applied to serum specimens collected from a smoking study with 24 volunteers and 212 forensic cases. Results are interpreted based upon the current knowledge about THC metabolism and pharmacokinetics.


    Author Information:

    Doerr, G

    Moeller, MR
    Professor, Institute of Legal Medicine, State University of Saarland, Homburg,

    Warth, S


    Stock #: JFS13282J

    ISSN: 0022-1198

    DOI: 10.1520/JFS13282J

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    Title Simultaneous Quantitation of Delta-9-tetrahydrocannabinol (THC) and 11-Nor-9-carboxy-delta-9-tetrahydrocannabinol (THC-COOH) in Serum by GC/MS Using Deuterated Internal Standards and Its Application to a Smoking Study and Forensic Cases
    Symposium , 0000-00-00
    Committee E30