Simultaneous Quantitation of Delta 9 tetrahydrocannabinol (THC) and 11 Nor 9 carboxy delta 9 tetrahydrocannabinol (THC COOH) in Serum by GC/MS Using Deuterated Internal Standards and Its Application to a Smoking Study and Forensic Cases

Volume 37, Issue 4 (July 1992)

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ISSN: 0022-1198
CODEN: JFSCA
Page Count: 15

Simultaneous Quantitation of Delta-9-tetrahydrocannabinol (THC) and 11-Nor-9-carboxy-delta-9-tetrahydrocannabinol (THC-COOH) in Serum by GC/MS Using Deuterated Internal Standards and Its Application to a Smoking Study and Forensic Cases
Doerr, G

Moeller, MR
Professor, Institute of Legal Medicine, State University of Saarland,

Warth, S

(Received 16 October 1991; accepted 18 November 1991)

Abstract

A new procedure for the simultaneous detection of delta-9-tetrahydrocannabinol (THC) and its major metabolite, 11-nor-9-carboxy-delta-9-tetrahydrocannabinol (THC-COOH) in serum has been evaluated. The method combines rapid, efficient, solid-phase extraction and simple derivatization by methylation. Analysis and quantitation is performed by gas chromatography/mass spectrometry (GC/MS) using deuterated cannabinoids as internal standards (IS). Reproducibility and sensitivity of the method are good.

The procedure is applied to serum specimens collected from a smoking study with 24 volunteers and 212 forensic cases. Results are interpreted based upon the current knowledge about THC metabolism and pharmacokinetics.

Keywords:
toxicology, cannabinoids, gas chromatography/mass spectrometry

Paper ID: JFS13282J
DOI: 10.1520/JFS13282J
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Author Title Simultaneous Quantitation of Delta-9-tetrahydrocannabinol (THC) and 11-Nor-9-carboxy-delta-9-tetrahydrocannabinol (THC-COOH) in Serum by GC/MS Using Deuterated Internal Standards and Its Application to a Smoking Study and Forensic Cases Symposium , 0000-00-00 Committee E30

		SEDL / Journals / Journal of Forensic Sciences (JOFS) / Citation Page Volume 37, Issue 4 (July 1992) Click here to download this paper now for $25 PDF (464K) View License Agreement ISSN: 0022-1198 CODEN: JFSCA Page Count: 15 Simultaneous Quantitation of Delta-9-tetrahydrocannabinol (THC) and 11-Nor-9-carboxy-delta-9-tetrahydrocannabinol (THC-COOH) in Serum by GC/MS Using Deuterated Internal Standards and Its Application to a Smoking Study and Forensic Cases Doerr, G Moeller, MR Professor, Institute of Legal Medicine, State University of Saarland, Warth, S (Received 16 October 1991; accepted 18 November 1991) Abstract A new procedure for the simultaneous detection of delta-9-tetrahydrocannabinol (THC) and its major metabolite, 11-nor-9-carboxy-delta-9-tetrahydrocannabinol (THC-COOH) in serum has been evaluated. The method combines rapid, efficient, solid-phase extraction and simple derivatization by methylation. Analysis and quantitation is performed by gas chromatography/mass spectrometry (GC/MS) using deuterated cannabinoids as internal standards (IS). Reproducibility and sensitivity of the method are good. The procedure is applied to serum specimens collected from a smoking study with 24 volunteers and 212 forensic cases. Results are interpreted based upon the current knowledge about THC metabolism and pharmacokinetics. Keywords: toxicology, cannabinoids, gas chromatography/mass spectrometry Paper ID: JFS13282J DOI: 10.1520/JFS13282J ASTM International is a member of CrossRef. Author Title Simultaneous Quantitation of Delta-9-tetrahydrocannabinol (THC) and 11-Nor-9-carboxy-delta-9-tetrahydrocannabinol (THC-COOH) in Serum by GC/MS Using Deuterated Internal Standards and Its Application to a Smoking Study and Forensic Cases Symposium , 0000-00-00 Committee E30