Journal Published Online: 01 July 1992
Volume 37, Issue 4

Simultaneous Quantitation of Delta-9-tetrahydrocannabinol (THC) and 11-Nor-9-carboxy-delta-9-tetrahydrocannabinol (THC-COOH) in Serum by GC/MS Using Deuterated Internal Standards and Its Application to a Smoking Study and Forensic Cases

CODEN: JFSCAS

Abstract

A new procedure for the simultaneous detection of delta-9-tetrahydrocannabinol (THC) and its major metabolite, 11-nor-9-carboxy-delta-9-tetrahydrocannabinol (THC-COOH) in serum has been evaluated. The method combines rapid, efficient, solid-phase extraction and simple derivatization by methylation. Analysis and quantitation is performed by gas chromatography/mass spectrometry (GC/MS) using deuterated cannabinoids as internal standards (IS). Reproducibility and sensitivity of the method are good.

The procedure is applied to serum specimens collected from a smoking study with 24 volunteers and 212 forensic cases. Results are interpreted based upon the current knowledge about THC metabolism and pharmacokinetics.

Author Information

Moeller, MR
Institute of Legal Medicine, State University of Saarland, Homburg, Germany
Doerr, G
Warth, S
Pages: 15
Price: $25.00
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Stock #: JFS13282J
ISSN: 0022-1198
DOI: 10.1520/JFS13282J