ISSN: 0022-1198
CODEN: JFSCA
Page Count: 7

Isoelectric Focusing of Human Hair Keratins: Correlation with Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis (SDS-PAGE) Patterns and Effect of Cosmetic Treatments
Carracedo, A
University of Santiago de Compostela,

Muñoz, I
University of Santiago de Compostela,

Concheiro, L
University of Santiago de Compostela,

Rodriguez-Calvo, MS
University of Santiago de Compostela,

(Received 31 May 1991; accepted 23 September 1991)

Abstract

A new isoelectric focusing (IEF) technique in polyacrylamide gels with 6M urea and 1.5% Nonidet P40 has been developed to characterize human hair samples. The phenotypes demonstrated with this procedure has been correlated with the sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) patterns described by other authors. The method described can be applied in the forensic science analysis of a single human hair. Using the same IEF technique we have studied the changes in electrophoretic patterns of cosmetically treated hair. The characteristics of the modifications observed and its utility in forensic science work are also discussed in this paper.

Keywords:
pathology and biology, hair, keratins, isoelectric focusing, noncarboxymethylated keratins

Paper ID: JFS13251J
DOI: 10.1520/JFS13251J
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