Isoelectric Focusing of Human Hair Keratins: Correlation with Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis (SDS-PAGE) Patterns and Effect of Cosmetic Treatments

    Volume 37, Issue 2 (March 1992)

    ISSN: 0022-1198

    CODEN: JFSOAD

    Page Count: 7


    Carracedo, A
    University of Santiago de Compostela, Galicia,

    Muñoz, I
    University of Santiago de Compostela, Galicia,

    Concheiro, L
    University of Santiago de Compostela, Galicia,

    Rodriguez-Calvo, MS
    University of Santiago de Compostela, Galicia,

    (Received 31 May 1991; accepted 23 September 1991)

    Abstract

    A new isoelectric focusing (IEF) technique in polyacrylamide gels with 6M urea and 1.5% Nonidet P40 has been developed to characterize human hair samples. The phenotypes demonstrated with this procedure has been correlated with the sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) patterns described by other authors. The method described can be applied in the forensic science analysis of a single human hair. Using the same IEF technique we have studied the changes in electrophoretic patterns of cosmetically treated hair. The characteristics of the modifications observed and its utility in forensic science work are also discussed in this paper.


    Paper ID: JFS13251J

    DOI: 10.1520/JFS13251J

    ASTM International
    is a member of CrossRef.

    Author
    Title Isoelectric Focusing of Human Hair Keratins: Correlation with Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis (SDS-PAGE) Patterns and Effect of Cosmetic Treatments
    Symposium , 0000-00-00
    Committee E30