Ribosomal Ribonucleic Acid (rRNA) Gene Typing for Species Identification

    Volume 37, Issue 2 (March 1992)

    ISSN: 0022-1198

    CODEN: JFSOAD

    Page Count: 8


    Naito, E
    Research associates and professor, Niigata University School of Medicine, Niigata,

    Ymanouchi, H
    Research associates and professor, Niigata University School of Medicine, Niigata,

    Dewa, K
    Research associates and professor, Niigata University School of Medicine, Niigata,

    Kominami, R
    Professor, Niigata University School of Medicine, Niigata,

    (Received 6 May 1991; accepted 19 August 1991)

    Abstract

    Deoxyribonucleic acid (DNA) typing of ribosomal ribonucleic acid (rRNA) genes was performed with a polymerase chain reaction (PCR) assay for species identification. A variable region of the 28S ribosomal RNA gene was amplified with primers complementary to flanking sequences phylogenetically well conserved. The products of twelve animal DNAs (human, Japanese monkey, dog, cattle, pig, cat, rabbit, mouse, rat, chicken, frog, and fish) were separated by polyacrylamide gel electrophoresis, each revealing a few bands ranging from 150 to 100 base pairs. The band patterns obtained from each DNA sample differed in number and size, which indicates the applicability of the method to species identification. Samples containing either as little as 1 pg of DNA or degraded DNA of 0.2 to 0.5 kb in length were able to give detectable bands. Postmorten human tissue DNAs were tested as an example. They showed a pattern identicial to the human control one, which was distinct from those of the other animals examined.


    Paper ID: JFS13249J

    DOI: 10.1520/JFS13249J

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    Author
    Title Ribosomal Ribonucleic Acid (rRNA) Gene Typing for Species Identification
    Symposium , 0000-00-00
    Committee E30