Volume 37, Issue 2 (March 1992)

    Isolation of Deoxyribonucleic Acid (DNA) from Saliva and Forensic Science Samples Containing Saliva

    (Received 25 October 1990; accepted 30 July 1991)

    CODEN: JFSOAD

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    Abstract

    Saliva and saliva-stained materials were examined as potential sources of deoxyribonucleic acid (DNA) for DNA analysis and identity testing. In this paper, the authors demonstrate that DNA was isolated and DNA banding patterns suitable for DNA typing were obtained from fresh saliva and various saliva-stained materials, such as envelopes, buccal swabs, gags, and cigarettes. Furthermore, DNA and DNA banding patterns were obtained from actual forensic evidentiary samples containing mixed saliva/semen stains. The DNA banding patterns obtained from saliva or saliva-stained material were indistinguishable from the patterns obtained from blood or hair from the same individual. Intact DNA was readily isolated and DNA banding patterns were obtained from saliva stored at −20°C and dried saliva stains stored under varying conditions. We conclude that saliva and saliva-stained material can be good sources of DNA for analysis and for DNA typing in certain forensic settings.


    Author Information:

    Corey, AC
    Staff molecular biologist, laboratory supervisor, deputy director, scientist, scientist and laboratory director, Cellmark Diagnostics, Germantown, MD

    Word, CJ
    Staff molecular biologist, laboratory supervisor, deputy director, scientist, scientist and laboratory director, Cellmark Diagnostics, Germantown, MD

    Garner, DD
    Staff molecular biologist, laboratory supervisor, deputy director, scientist, scientist and laboratory director, Cellmark Diagnostics, Germantown, MD

    Walsh, DJ
    Staff molecular biologist, laboratory supervisor, deputy director, scientist, scientist and laboratory director, Cellmark Diagnostics, Germantown, MD

    Herrin, GL
    Georgia Bureau of Investigation, Decatur, GA

    Forman, L
    Staff molecular biologist, laboratory supervisor, deputy director, scientist, scientist and laboratory director, Cellmark Diagnostics, Germantown, MD

    Cotton, RW
    Staff molecular biologist, laboratory supervisor, deputy director, scientist, scientist and laboratory director, Cellmark Diagnostics, Germantown, MD


    Stock #: JFS13248J

    ISSN: 0022-1198

    DOI: 10.1520/JFS13248J

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    Title Isolation of Deoxyribonucleic Acid (DNA) from Saliva and Forensic Science Samples Containing Saliva
    Symposium , 0000-00-00
    Committee E30