ISSN: 0022-1198
CODEN: JFSCA
Page Count: 5

An Improved Method for Subtyping Pi in Old Bloodstains
Gremo, A
Associate investigators, titular professor, and chief, University Complutense,

Arroyo, E
Associate investigators, titular professor, and chief, University Complutense,

López-Abadía, I
Associate investigators, titular professor, and chief, University Complutense,

Ruiz de la Cuesta, JM
Associate investigators, titular professor, and chief, University Complutense,

(Received 11 May 1991; accepted 22 July 1991)

Abstract

Immunofixation procedures were used for detecting alpha-1 antitrypsin protease inhibitor (Pi) phenotypes in bloodstains. Neuraminidase elution of bloodstains, together with isoelectric focusing, immunofixation, and silver staining techniques, makes possible Pi subtyping in old bloodstains. No extra bands appear when the storage time is no longer than three months.

Keywords:
pathology and biology, genetic typing, human identification, bloodstains, alpha-1 antitrypsin, immunofixation, silver staining, protease inhibitor

Paper ID: JFS13236J
DOI: 10.1520/JFS13236J
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