Journal Published Online: 01 January 1992
Volume 37, Issue 1

Analysis of Buprenorphine in Urine Specimens

CODEN: JFSCAS

Abstract

The simultaneous determination of buprenorphine (Temgesic®) and its major metabolite, N-desalkylbuprenorphine, in urine samples has been studied. By using reversed-phase high-performance liquid chromatography (HPLC) with electrochemical detection, therapeutic concentrations of unconjugated buprenorphine down to 0.2 ng/mL, and 0.15 ng/mL for the metabolite, can be detected in urine samples. This method has been applied to a variety of urine samples from drug users. The possible analytical interference from several other regulated drugs has been studied. The results were also compared with those obtained from a commercial radioimmunoassay (RIA) test. This test is only capable of detecting buprenorphine concentrations higher than 1 ng/mL.

Author Information

Debrabandere, L
Laboratory of Toxicology, University of Louvain, Leuven, Belgium
Van Boven, M
Laboratory of Toxicology, University of Louvain, Leuven, Belgium
Daenens, P
Laboratory of Toxicology, University of Louvain, Leuven, Belgium
Pages: 8
Price: $25.00
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Stock #: JFS13214J
ISSN: 0022-1198
DOI: 10.1520/JFS13214J