ISSN: 0022-1198
CODEN: JFSCA
Page Count: 11

A Single-Column Procedure on Bond Elut Certify for Systematic Toxicological Analysis of Drugs in Plasma and Urine
Franke, J-P
Research associates, senior research associate, and professor of toxicology, University Centre for Pharmacy,

Wijsbeek, J
Research associates, senior research associate, and professor of toxicology, University Centre for Pharmacy,

de Zeeuw, RA
Research associates, senior research associate, and professor of toxicology, University Centre for Pharmacy,

Chen, X-H
Research associates, senior research associate, and professor of toxicology, University Centre for Pharmacy,

(Received 16 March 1991; accepted 4 June 1991)

Abstract

A single-column solid-phase extraction procedure was developed for the screening of acidic, neutral, and basic drugs from plasma. The recoveries of all 25 tested drugs exceeded 82%. After the plasma had been diluted with phosphate buffer (pH 6.0), the drugs were extracted using a single Bond Elut Certify column. The acidic and most of the neutral drugs were eluted by acetone/chloroform (1:1) and the basic drugs were eluted by 2% ammoniated ethyl acetate. Some neutral drugs appeared in both fractions. The two fractions were collected separately and evaporated until approximately 100 μL of solvent remained in the tube. Both fractions were analyzed separately on a gas chromatograph equipped with a wide-bore capillary column and a flame ionization detector. The procedure could also be used for urine samples.

Keywords:
toxicology, extraction, drug identification, blood, solid-phase extraction, plasma, urine, systematic toxicological analysis

Paper ID: JFS13211J
DOI: 10.1520/JFS13211J
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