Volume 37, Issue 1 (January 1992)

    A Polymerase Chain Reaction (PCR) Method for Sex and Species Determination with Novel Controls for Deoxyribonucleic Acid (DNA) Template Length

    (Received 12 June 1991; accepted 27 June 1991)

    CODEN: JFSOAD

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    Abstract

    Human X and Y chromosome α-satellite sequences lying within higher order repeats were amplified by the polymerase chain reaction (PCR) in genomic deoxyribonucleic acid (DNA) isolated from blood, bone, and several other tissues and specimens of potential forensic science interest. X and Y sequences could be coamplified under some of the PCR conditions employed. Monomorphic sequences in the 3′-apolipoprotein B gene (designated “H”) and in an α-satellite higher order repeat on Chromosome 17 (p17H8, D17Z1) were likewise amplified in the specimens. X and Y sequence amplification can provide information about the sex of origin. Amplification of the X, H, and D17Z1 sequences was found to be primate-specific among the common animals tested and can thus provide species of origin information about a specimen. The authors suggest that amplification of X and D17Z1 or H sequences might provide “relaxed” and “stringent” controls for appropriate PCR amplification tests on forensic science specimens. Testing was carried out using PCR protocols that employed Thermophilus aquaticus (Taq) and Thermus flavis (Replinase) thermostable DNA polymerases.


    Author Information:

    Grosso, DA
    Professor and director, research associate, and graduate research assistant, Forensic Sciences Program and Laboratories, University of New Haven, West Haven, CT

    Messina, D
    Research associate, supervisory criminalist, senior criminalist, and chief criminalist and director, Connecticut State Police Forensic Science Laboratory, Meriden, CT

    Pagliaro, EM
    Research associate, supervisory criminalist, senior criminalist, and chief criminalist and director, Connecticut State Police Forensic Science Laboratory, Meriden, CT

    Ruano, G
    Research associate, supervisory criminalist, senior criminalist, and chief criminalist and director, Connecticut State Police Forensic Science Laboratory, Meriden, CT

    Lee, HC
    Research associate, supervisory criminalist, senior criminalist, and chief criminalist and director, Connecticut State Police Forensic Science Laboratory, Meriden, CT

    Berka, KM
    Professor and director, research associate, and graduate research assistant, Forensic Sciences Program and Laboratories, University of New Haven, West Haven, CT

    Gaensslen, RE
    Professor and director, research associate, and graduate research assistant, Forensic Sciences Program and Laboratories, University of New Haven, West Haven, CT


    Stock #: JFS13207J

    ISSN: 0022-1198

    DOI: 10.1520/JFS13207J

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    Title A Polymerase Chain Reaction (PCR) Method for Sex and Species Determination with Novel Controls for Deoxyribonucleic Acid (DNA) Template Length
    Symposium , 0000-00-00
    Committee E30