Typing of Deoxyribonucleic Acid (DNA) Extracted from Compact Bone from Human Remains

    Volume 36, Issue 6 (November 1991)

    ISSN: 0022-1198

    CODEN: JFSOAD

    Page Count: 13


    Budowle, B
    Research chemists, Forensic Science Research and Training Center, Laboratory Division, FBI Academy, Quantico, VA

    Comey, CT
    Research chemists, Forensic Science Research and Training Center, Laboratory Division, FBI Academy, Quantico, VA

    Hochmeister, MN
    Forensic scientists, research assistant, and director, Institut für Rechtsmedizin, University of Bern, Bern,

    Dirnhofer, R
    Forensic scientists, research assistant, and director, Institut für Rechtsmedizin, University of Bern, Bern,

    Borer, UV
    Forensic scientists, research assistant, and director, Institut für Rechtsmedizin, University of Bern, Bern,

    Eggmann, U
    Forensic scientists, research assistant, and director, Institut für Rechtsmedizin, University of Bern, Bern,

    (Received 30 March 1991; accepted 14 May 1991)

    Abstract

    The application of deoxyribonucleic acid (DNA) typing methods for the potential identification of unknown human remains was investigated. DNA was isolated from compact bone tissue from badly decomposed bodies and from known and unknown human remains, using a decalcification and ion wash procedure. Restriction fragment length polymorphism (RFLP) analysis of variable number of tandem repeats (VNTR) loci yielded results in some cases, but more often the DNA was too degraded to produce RFLP patterns. No RFLP profiles could be obtained from putrified soft tissues.

    However, DNA extracted from compact bone tissue of human remains up to eleven years old was successfully amplified using the polymerase chain reaction (PCR) for the VNTR loci D1S80, D17S5, COL2A1, and APO B, as well as the HLA-DQ alpha locus. This is especially significant, since PCR results were obtained from those samples whose DNA had been degraded substantially and had yielded no RFLP patterns. All DNA types determined from the compact bone tissue from decomposed bodies whose identification had been established first by other means (and whose parents or offspring were available for typing) demonstrated mendelian inheritance of the alleles of the loci analyzed. These results suggest that amplification and typing of DNA extracted from compact bone of human remains could be useful in establishing the identity of a person, as well as in excluding possible false identifications.


    Paper ID: JFS13189J

    DOI: 10.1520/JFS13189J

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    Title Typing of Deoxyribonucleic Acid (DNA) Extracted from Compact Bone from Human Remains
    Symposium , 0000-00-00
    Committee E30