Simultaneous Analysis of Amphetamine, Methamphetamine, and 3,4-Methylenedioxymethamphetamine (MDMA) in Urine Samples by Solid-Phase Extraction, Derivatization, and Gas Chromatography/Mass Spectrometry

    Volume 36, Issue 5 (September 1991)

    ISSN: 0022-1198

    CODEN: JFSOAD

    Page Count: 11


    Walia, AS
    Director, Environmental Health Research and Testing, Inc., Birmingham, AL

    Gan, BK
    Graduate students and professor, University of Alabama at Birmingham, Birmingham, AL

    Baugh, D
    Graduate students and professor, University of Alabama at Birmingham, Birmingham, AL

    Liu, RH
    Graduate students and professor, University of Alabama at Birmingham, Birmingham, AL

    (Received 31 October 1990; accepted 26 February 1991)

    Abstract

    A rapid and effective solid-phase extraction procedure using Bond Elute Certify™ bonded silica sorbent cartridges was adopted to extract amphetamine, methamphetamine, and 3,4-methylenedioxymethamphetamine (MDMA or Ecstasy) from urine samples. The extract was derivatized with trichloroacetic anhydride prior to gas chromatography/mass spectrometry (GC/MS) analysis with selected ion monitoring of the following ions: 190, 91, 188; 204, 91, 202; 162, 135, 202; 194, 123; and 211, 209 for the derivatized amphetamine, methamphetamine, MDMA, d5-amphetamine, and d9-methamphetamine, respectively. The first of the ions listed for each compound was used for quantitation. The compound d5-amphetamine was used as the internal standard for amphetamine, and d9-methamphetamine was used for methamphetamine and MDMA. Results showed a higher than 65% recovery and a reproducibility with less than a 5% coefficient of variation. When a sample size of 2 mL was used, the lowest detectable concentration was about 50 ng/mL, and a near-perfect fit can be obtained (within the 250 to 4000-ng/mL concentration range studied) using a second-order polynomial model.


    Paper ID: JFS13155J

    DOI: 10.1520/JFS13155J

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    Title Simultaneous Analysis of Amphetamine, Methamphetamine, and 3,4-Methylenedioxymethamphetamine (MDMA) in Urine Samples by Solid-Phase Extraction, Derivatization, and Gas Chromatography/Mass Spectrometry
    Symposium , 0000-00-00
    Committee E30