ISSN: 0022-1198
CODEN: JFSCA
Page Count: 9

Fixed Human Tissues: A Resource for the Identification of Individuals
Shibata, D
Assistant professor of pathology, University of Southern California School of Medicine and Los Angeles County + University of Southern California Medical Center, CA

Kurosu, M
Visiting scholar in forensic medicine from the Department of Legal Medicine, Nippon Medical School,

Noguchi, TT
Professor of pathology, University of Southern California School of Medicine and Los Angeles County + University of Southern California Medical Center, CA

(Received 10 July 1990; accepted 26 October 1990)

Abstract

Polymorphic genetic loci of the deoxyribonucleic acid (DNA) present in formalin-fixed, paraffin-embedded tissues were successfully analyzed by utilizing the polymerase chain reaction. Using this analysis, with three different polymorphic loci [human leucocyte antigen (HLA) DQ alpha, low-density lipoprotein receptor, and parathyroid hormone], fixed tissues representing 14 different individuals were genotyped and could be distinguished from each other. The techniques were further applied to the fixed autopsy tissues of a man in which a question of paternity arose postmortem. Since many individuals have surgical procedures or autopsy, these readily available fixed tissues represent an additional resource for the identification of individuals.

Keywords:
pathology and biology, tissues (biology), human identification, genetic typing, polymerase chain reaction, forensic identification, paraffin-embedded tissue, human leucocyte antigen, paternity testing, deoxyribonucleic acid (DNA) typing

Paper ID: JFS13137J
DOI: 10.1520/JFS13137J
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