Journal Published Online: 01 July 1991
Volume 36, Issue 4

Fixed Human Tissues: A Resource for the Identification of Individuals

CODEN: JFSCAS

Abstract

Polymorphic genetic loci of the deoxyribonucleic acid (DNA) present in formalin-fixed, paraffin-embedded tissues were successfully analyzed by utilizing the polymerase chain reaction. Using this analysis, with three different polymorphic loci [human leucocyte antigen (HLA) DQ alpha, low-density lipoprotein receptor, and parathyroid hormone], fixed tissues representing 14 different individuals were genotyped and could be distinguished from each other. The techniques were further applied to the fixed autopsy tissues of a man in which a question of paternity arose postmortem. Since many individuals have surgical procedures or autopsy, these readily available fixed tissues represent an additional resource for the identification of individuals.

Author Information

Shibata, D
University of Southern California School of Medicine and Los Angeles County + University of Southern California Medical Center, Los Angeles, CA
Kurosu, M
Nippon Medical School, Tokyo, Japan
Noguchi, TT
University of Southern California School of Medicine and Los Angeles County + University of Southern California Medical Center, Los Angeles, CA
Pages: 9
Price: $25.00
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Stock #: JFS13137J
ISSN: 0022-1198
DOI: 10.1520/JFS13137J