Sensitive and Specific Quantification of Human Genomic Deoxyribonucleic Acid (DNA) in Forensic Science Specimens: Casework Examples

    Volume 36, Issue 4 (July 1991)

    ISSN: 0022-1198

    CODEN: JFSOAD

    Page Count: 6


    Michaud, D
    Serology specialist and molecular genetics specialist, Central Forensic Laboratory, Royal Canadian Mounted Police, Ottawa,

    Bowen, JH
    Serology specialist and molecular genetics specialist, Central Forensic Laboratory, Royal Canadian Mounted Police, Ottawa,

    Waye, JS
    Assistant professor of pathology, McMaster University, Hamilton, Ontario

    Fourney, RM
    Adjunct professor, University of Ottawa, Ottawa, Ontario

    (Received 10 October 1990; accepted 26 October 1990)

    Abstract

    We describe the forensic science application of a method for quantification of human genomic deoxyribonucleic acid (DNA). The two cases cited in this report involve DNA samples extracted from skin tissue and bloodstained clothing recovered from different crime scenes. High-molecular-weight DNA was recovered from both specimens, and the concentrations of these DNAs were estimated to be ∼0.5 µg/µL by ethidium bromide/agarose gel electrophoresis. Using the human-specific DNA probe p17H8 (locus D17Z1) to quantify the amount of human genomic DNA in these samples, it is shown that less than 1% of the DNA isolated from the skin tissue is of human origin and that the DNA isolated from the bloodstained clothing is effectively devoid of human DNA sequences. These case examples illustrate the need to quantify not only the total amount of DNA recovered from forensic casework material, but also the proportion of the DNA that is of human origin.


    Paper ID: JFS13136J

    DOI: 10.1520/JFS13136J

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    Title Sensitive and Specific Quantification of Human Genomic Deoxyribonucleic Acid (DNA) in Forensic Science Specimens: Casework Examples
    Symposium , 0000-00-00
    Committee E30