ISSN: 0022-1198
CODEN: JFSCA
Page Count: 8

Simultaneous Gas Chromatography/Mass Spectrometry Assay of Methadone and 2-Ethyl-1, 5-Dimethyl-3,3-Diphenylpyrrolidine (EDDP) in Urine
Walia, AS
Chief, Alcoholism Research, VA Medical Center, and research associate professor, School of Medicine, University of Alabama at Birmingham, AL

Baugh, LD
University of Alabama at Birmingham, AL

Liu, RH
University of Alabama at Birmingham, AL

(Received 31 October 1989; accepted 15 May 1990)

Abstract

An efficient extraction and gas chromatography/mass spectrometry (GC/MS) procedure has been developed for the simultaneous determination of methadone and 2-ethyl-1, 5-dimethyl-3,3-diphenylpyrrolidine in urine samples. The merits of this procedure include (1) effective high-volume sample processing; (2) excellent gas chromatography characteristics; (3) high precision for quantitative methadone determination—1.0% coefficient of variation (CV) for GC/MS injection replicates and 1.2% for extraction replicates; (4) excellent linearity within the range (0 to 1200 ng/mL) studied; and (5) adequate detection limits (50 ng/mL) for most practical purposes. The detection limit for methadone may be improved 40-fold by using a different internal standard.

Keywords:
toxicology, abuse drugs, 2-ethyl-1,5-dimethyl-3,3-diphenylpyrrolidine (EDDP), chemical analyses, urine, GC/MS, methadone, selected ion monitoring, urinalysis

Paper ID: JFS13057J
DOI: 10.1520/JFS13057J
ASTM International is a member of CrossRef.