Genetic Markers in Human Bone: I. Deoxyribonucleic Acid (DNA) Analysis

    Volume 36, Issue 2 (March 1991)

    ISSN: 0022-1198

    CODEN: JFSOAD

    Page Count: 11


    Garner, DD
    Staff scientist and laboratory director, Cellmark Diagnostics, Germantown, MD

    Anderson, DT
    Research associate, laboratory coordinator, graduate research assistant, and professor and director, Forensic Sciences Program and Laboratories, University of New Haven, West Haven, CT

    Pagliaro, EM
    Chief criminalist and director, supervisory criminalist, and research associate, Connecticut State Police Forensic Science Laboratory, Meriden, CT

    Herrin, GL
    Staff scientist and laboratory director, Cellmark Diagnostics, Germantown, MD

    Ruano, G
    Chief criminalist and director, supervisory criminalist, and research associate, Connecticut State Police Forensic Science Laboratory, Meriden, CT

    Lee, HC
    Chief criminalist and director, supervisory criminalist, and research associate, Connecticut State Police Forensic Science Laboratory, Meriden, CT

    Berka, KM
    Research associate, laboratory coordinator, graduate research assistant, and professor and director, Forensic Sciences Program and Laboratories, University of New Haven, West Haven, CT

    Folk, NL
    Research associate, laboratory coordinator, graduate research assistant, and professor and director, Forensic Sciences Program and Laboratories, University of New Haven, West Haven, CT

    Phipps, P
    Manager Human Genetics Research and Technology Development, and staff scientist, Collaborative Research, Inc., Bedford, MA

    Gaensslen, RE
    Research associate, laboratory coordinator, graduate research assistant, and professor and director, Forensic Sciences Program and Laboratories, University of New Haven, West Haven, CT

    Keith, TP
    Manager Human Genetics Research and Technology Development, and staff scientist, Collaborative Research, Inc., Bedford, MA

    (Received 29 May 1990; accepted 12 June 1990)

    Abstract

    Deoxyribonucleic acid (DNA) was isolated from a number of spongy and compact human bone tissue specimens, and the yield was estimated on a “per milligram of starting tissue” basis. DNA was, in addition, isolated from a number of corresponding blood and bone tissue specimens. Spectrophotofluorometry and ethidium bromide visualization on minigels were used to estimate the quantity and degree of degradation of DNA. The DNA from several blood-bone pairs is shown to give concordant restriction fragment length polymorphism (RFLP) typing results by two different typing protocols with five different singlelocus probes. DNA from several additional blood-bone pairs is shown to give concordant results for human leucocyte antigen (HLA)-DQα phenotypes following polymerase chain reaction (PCR) amplification and hybridization to specific allele-specific oligonucleotide (ASO) probes, and for the variable numbers of tandem repeats (VNTR) length polymorphisms 3′ to the human apolipoprotein B (APOB) gene following PCR amplification with specific primers and analysis of the products by electrophoresis and ethidium bromide visualization.


    Paper ID: JFS13034J

    DOI: 10.1520/JFS13034J

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    Title Genetic Markers in Human Bone: I. Deoxyribonucleic Acid (DNA) Analysis
    Symposium , 0000-00-00
    Committee E30