ISSN: 0022-1198
CODEN: JFSCA
Page Count: 11

Genetic Markers in Human Bone: I. Deoxyribonucleic Acid (DNA) Analysis
Garner, DD
Staff scientist and laboratory director, Cellmark Diagnostics, MD

Anderson, DT
Research associate, laboratory coordinator, graduate research assistant, and professor and director, Forensic Sciences Program and Laboratories, University of New Haven, CT

Pagliaro, EM
Chief criminalist and director, supervisory criminalist, and research associate, Connecticut State Police Forensic Science Laboratory, CT

Herrin, GL
Staff scientist and laboratory director, Cellmark Diagnostics, MD

Ruano, G
Chief criminalist and director, supervisory criminalist, and research associate, Connecticut State Police Forensic Science Laboratory, CT

Lee, HC
Chief criminalist and director, supervisory criminalist, and research associate, Connecticut State Police Forensic Science Laboratory, CT

Berka, KM
Research associate, laboratory coordinator, graduate research assistant, and professor and director, Forensic Sciences Program and Laboratories, University of New Haven, CT

Folk, NL
Research associate, laboratory coordinator, graduate research assistant, and professor and director, Forensic Sciences Program and Laboratories, University of New Haven, CT

Phipps, P
Manager Human Genetics Research and Technology Development, and staff scientist, Collaborative Research, Inc., MA

Gaensslen, RE
Research associate, laboratory coordinator, graduate research assistant, and professor and director, Forensic Sciences Program and Laboratories, University of New Haven, CT

Keith, TP
Manager Human Genetics Research and Technology Development, and staff scientist, Collaborative Research, Inc., MA

(Received 29 May 1990; accepted 12 June 1990)

Abstract

Deoxyribonucleic acid (DNA) was isolated from a number of spongy and compact human bone tissue specimens, and the yield was estimated on a “per milligram of starting tissue” basis. DNA was, in addition, isolated from a number of corresponding blood and bone tissue specimens. Spectrophotofluorometry and ethidium bromide visualization on minigels were used to estimate the quantity and degree of degradation of DNA. The DNA from several blood-bone pairs is shown to give concordant restriction fragment length polymorphism (RFLP) typing results by two different typing protocols with five different singlelocus probes. DNA from several additional blood-bone pairs is shown to give concordant results for human leucocyte antigen (HLA)-DQα phenotypes following polymerase chain reaction (PCR) amplification and hybridization to specific allele-specific oligonucleotide (ASO) probes, and for the variable numbers of tandem repeats (VNTR) length polymorphisms 3′ to the human apolipoprotein B (APOB) gene following PCR amplification with specific primers and analysis of the products by electrophoresis and ethidium bromide visualization.

Keywords:
pathology and biology, deoxyribonucleic acid (DNA), genetic typing, forensic DNA typing, human bone DNA, bone DNA typing, bone genetic markers

Paper ID: JFS13034J
DOI: 10.1520/JFS13034J
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