Hair Analysis for Drug Abuse: I. Determination of Methamphetamine and Amphetamine in Hair by Stable Isotope Dilution Gas Chromatography/Mass Spectrometry Method

    Volume 36, Issue 1 (January 1991)

    ISSN: 0022-1198

    CODEN: JFSOAD

    Page Count: 9


    Takahashi, K
    Section chief and senior forensic chemists, Narcotics Section, National Institute of Hygienic Sciences, Tokyo,

    Shimamine, M
    Section chief and senior forensic chemists, Narcotics Section, National Institute of Hygienic Sciences, Tokyo,

    Nakahara, Y
    Section chief and senior forensic chemists, Narcotics Section, National Institute of Hygienic Sciences, Tokyo,

    Takeda, Y
    Head, Division of Drugs, National Institute of Hygienic Sciences, Tokyo,

    (Received 6 January 1990; accepted 14 February 1990)

    Abstract

    Determination of methamphetamine and amphetamine in hair was performed by gas chromatography/mass spectrometry using stable isotope-labeled internal standards, 2-methylamino-1-phenylpropane-2,3,3,3-d4 and 2-amino-1-phenylpropane-2,3,3,3-d4. Extraction of hair with methanol/5M hydrochloric acid (20:1) using ultrasonication was chosen as the standard method. The calibration curves for amphetamines in the hair were linear from 1 to 100 ng/mg (r > 0.99). The detection limit was 0.5 ng/mg at the 95% confidence level. The coefficients of variation (CV) (n = 8) of analysis using the spiked hair with methamphetamine were from 0.7 to 6%. The CV (n = 8) of analysis of the methamphetamine abuser's hair was 17.5%. Sectional analysis of monkey and human hair after methamphetamine ingestion suggested a good correlation between the duration of drug use and drug distribution in the hair.


    Paper ID: JFS13007J

    DOI: 10.1520/JFS13007J

    ASTM International
    is a member of CrossRef.

    Author
    Title Hair Analysis for Drug Abuse: I. Determination of Methamphetamine and Amphetamine in Hair by Stable Isotope Dilution Gas Chromatography/Mass Spectrometry Method
    Symposium , 0000-00-00
    Committee E30