Volume 35, Issue 6 (November 1990)
Comparison of Dyes from Transferred Fibers by Scanning Densitometry
The application of scanning densitometry to the analysis of dyes extracted from transferred fibers is examined. The Camag high-performance thin-layer chromatography (HPTLC)/thin-layer chromatography (TLC) scanner can provide all of the information normally available from visual examination of the TLC plates. The position, color, and relative proportions of the various components are obtained in a semiquantitative form. The instrument can provide useful data in both the visible and fluorescence modes with greater sensitivity than is available from visual observations. The potential of the instrument for examination of dyes from pale-colored fibers is demonstrated.
The data obtained are objective and do not suffer from the variations that can occur when using subjective visual observations.