(Received 2 December 1989; accepted 26 January 1990)
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The effects of endogenous 5-methylcytosines on deoxyribonucleic acid (DNA) fingerprints were studied. Analysis with methylation-sensitive restriction endonuclease Sau3AI and its methylation-insensitive isoschizomer MboI showed some differences in the patterns generated as a result of 5-methylcytosines at the recognition sites. Moreover, a few bands of sperm DNA did not match those of blood DNA from the same individual, a phenomenon only observed in the digests of methylation-sensitive endonucleases. These findings indicate the unsuitability of methylation-sensitive restriction endonucleases for DNA fingerprinting and other forms of DNA typing, because of the tissue-specific status of the methylation.
Research associate, Institute of Community Medicine, University of Tsukuba, Ibaraki,
Professor, Institute of Community Medicine, University of Tsukuba, Ibaraki,
Associate professor, Faculty of Science, The University of Tokyo, Tokyo,
Stock #: JFS12961J