ISSN: 0022-1198
CODEN: JFSCA
Page Count: 7

Effects of Cytosine Methylation at Restriction Sites on Deoxyribonucleic Acid (DNA) Typing
Washio, K
Research associate, Institute of Community Medicine, University of Tsukuba,

Misawa, S
Professor, Institute of Community Medicine, University of Tsukuba,

Ueda, S
Associate professor, Faculty of Science, The University of Tokyo,

(Received 2 December 1989; accepted 26 January 1990)

Abstract

The effects of endogenous 5-methylcytosines on deoxyribonucleic acid (DNA) fingerprints were studied. Analysis with methylation-sensitive restriction endonuclease Sau3AI and its methylation-insensitive isoschizomer MboI showed some differences in the patterns generated as a result of 5-methylcytosines at the recognition sites. Moreover, a few bands of sperm DNA did not match those of blood DNA from the same individual, a phenomenon only observed in the digests of methylation-sensitive endonucleases. These findings indicate the unsuitability of methylation-sensitive restriction endonucleases for DNA fingerprinting and other forms of DNA typing, because of the tissue-specific status of the methylation.

Keywords:
forensic science, deoxyribonucleic acid (DNA), genetic typing

Paper ID: JFS12961J
DOI: 10.1520/JFS12961J
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