Effects of Cytosine Methylation at Restriction Sites on Deoxyribonucleic Acid (DNA) Typing

    Volume 35, Issue 6 (November 1990)

    ISSN: 0022-1198

    CODEN: JFSOAD

    Page Count: 7


    Washio, K
    Research associate, Institute of Community Medicine, University of Tsukuba, Ibaraki,

    Misawa, S
    Professor, Institute of Community Medicine, University of Tsukuba, Ibaraki,

    Ueda, S
    Associate professor, Faculty of Science, The University of Tokyo, Tokyo,

    (Received 2 December 1989; accepted 26 January 1990)

    Abstract

    The effects of endogenous 5-methylcytosines on deoxyribonucleic acid (DNA) fingerprints were studied. Analysis with methylation-sensitive restriction endonuclease Sau3AI and its methylation-insensitive isoschizomer MboI showed some differences in the patterns generated as a result of 5-methylcytosines at the recognition sites. Moreover, a few bands of sperm DNA did not match those of blood DNA from the same individual, a phenomenon only observed in the digests of methylation-sensitive endonucleases. These findings indicate the unsuitability of methylation-sensitive restriction endonucleases for DNA fingerprinting and other forms of DNA typing, because of the tissue-specific status of the methylation.


    Paper ID: JFS12961J

    DOI: 10.1520/JFS12961J

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    Title Effects of Cytosine Methylation at Restriction Sites on Deoxyribonucleic Acid (DNA) Typing
    Symposium , 0000-00-00
    Committee E30