Journal Published Online: 01 September 1990
Volume 35, Issue 5

Chemically Sensitized Erythrocytes for Hemagglutination Reactions

CODEN: JFSCAS

Abstract

The use of chemically modified indicator erythrocytes for hemagglutination reactions can result in increased sensitivity. Treatment of erythrocytes with polyvinylpyrrolidone (PVP) or dextran T40 (10% weight/volume) induces changes in the cell surface in the form of extensions and blebbing, thereby increasing the surface area. These sensitized cells can be used in forensic science when detection or quantitation of erythrocyte surface reacting antibodies is important. The effect of altering membrane lipid fluidity on erythrocyte surface antigens has also been investigated. Treatment of cells with a reagent that increases the membrane ratio of cholesterol to phospholipid results in enhanced hemagglutination capacity despite the lack of extensive spiculation.

Author Information

Harrington, JJ
New Jersey State Police, North Regional Laboratory, Little Falls, NJ
Gordon, RE
Mount Sinai School of Medicine, New York, NY
Ross, JP
John Jay College of Criminal Justice, City University of New York, New York, NY
Kobilinsky, L
Doctoral Faculty Biochemistry, City University Graduate Center, City University of New York, New York, NY
Pages: 10
Price: $25.00
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Stock #: JFS12933J
ISSN: 0022-1198
DOI: 10.1520/JFS12933J