Volume 35, Issue 4 (July 1990)

    Enhanced Conditions for DNA Fingerprinting with Biotinylated M13 Bacteriophage

    (Received 30 May 1989; accepted 6 September 1989)

    CODEN: JFSOAD

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    Abstract

    Deoxyribonucleic acid (DNA) fingerprints are Southern blots which have a pattern resembling bar codes. The pattern is created by DNA probes that bind to variable-length repeated sequences of human genomic DNA digested with restriction endonucleases. To improve DNA fingerprints obtained with biotin-labeled M13mp8 replicative form (RF) bacteriophage as the gene probe, the conditions for hybridization and the subsequent washing steps of the filter were refined. Experiments were conducted varying the electrophoresis time, blotting membranes, hybridization solution, and posthybridization washes. The simplicity, sensitivity, and reliability of this nonisotopic technique make possible its application for identification of individuals within a species, for parentage testing, and for monitoring bone marrow transplantation.


    Author Information:

    Booth, F
    Forensic chemist, Regional Crime Laboratory, Kansas City, MO

    Tilzer, LL
    Postdoctoral fellow, medical student, and associate professor, Kansas University Medical Center, Kansas City, KS

    Moreno, RF
    Postdoctoral fellow, medical student, and associate professor, Kansas University Medical Center, Kansas City, KS

    Thomas, SM
    Postdoctoral fellow, medical student, and associate professor, Kansas University Medical Center, Kansas City, KS


    Stock #: JFS12895J

    ISSN: 0022-1198

    DOI: 10.1520/JFS12895J

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    Title Enhanced Conditions for DNA Fingerprinting with Biotinylated M13 Bacteriophage
    Symposium , 0000-00-00
    Committee E30