ISSN: 0022-1198
CODEN: JFSCA
Page Count: 7

Enhanced Conditions for DNA Fingerprinting with Biotinylated M13 Bacteriophage
Booth, F
Forensic chemist, Regional Crime Laboratory, MO

Tilzer, LL
Postdoctoral fellow, medical student, and associate professor, Kansas University Medical Center, KS

Moreno, RF
Postdoctoral fellow, medical student, and associate professor, Kansas University Medical Center, KS

Thomas, SM
Postdoctoral fellow, medical student, and associate professor, Kansas University Medical Center, KS

(Received 30 May 1989; accepted 6 September 1989)

Abstract

Deoxyribonucleic acid (DNA) fingerprints are Southern blots which have a pattern resembling bar codes. The pattern is created by DNA probes that bind to variable-length repeated sequences of human genomic DNA digested with restriction endonucleases. To improve DNA fingerprints obtained with biotin-labeled M13mp8 replicative form (RF) bacteriophage as the gene probe, the conditions for hybridization and the subsequent washing steps of the filter were refined. Experiments were conducted varying the electrophoresis time, blotting membranes, hybridization solution, and posthybridization washes. The simplicity, sensitivity, and reliability of this nonisotopic technique make possible its application for identification of individuals within a species, for parentage testing, and for monitoring bone marrow transplantation.

Keywords:
pathology and biology, deoxyribonucleic acid (DNA), bacteriophage

Paper ID: JFS12895J
DOI: 10.1520/JFS12895J
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