Volume 35, Issue 4 (July 1990)

    Validity Testing of Commercial Urine Cocaine Metabolite Assays: IV. Evaluation of the EMIT® d.a.u.™ Cocaine Metabolite Assay in a Quantitative Mode for Detection of Cocaine Metabolite

    (Received 1 July 1989; accepted 20 July 1989)

    CODEN: JFSOAD

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    Abstract

    The EMIT® d.a.u.™ cocaine metabolite assay (EMIT dau) was evaluated in a quantitative mode for analysis of clinical specimens obtained after controlled cocaine administration to human subjects. The quantitative results showed high concordance with those of gas chromatography/mass spectrometry (GC/MS) assays of the same specimens for benzoylecgonine, and no false positive or false negative results were obtained. The evaluation also included analysis of standardized solutions containing benzoylecgonine, cocaine, and other cocaine metabolites and isomers. The EMIT dau antibody demonstrated high selectivity for benzoylecgonine. The precision was somewhat less than that reported earlier for other commercial cocaine metabolite immunoassays. Quantitation of initial screening results from EMIT dau testing can serve as a useful guide for confirmation by GC/MS in forensic science urine testing.


    Author Information:

    Yousefnejad, D
    Chief, chemist, and medical technologist (ASCP), Laboratory of Chemistry and Drug Metabolism, Addiction Research Center, National Institute on Drug Abuse, Baltimore, MD

    Cone, EJ
    Chief, chemist, and medical technologist (ASCP), Laboratory of Chemistry and Drug Metabolism, Addiction Research Center, National Institute on Drug Abuse, Baltimore, MD

    Dickerson, SL
    Chief, chemist, and medical technologist (ASCP), Laboratory of Chemistry and Drug Metabolism, Addiction Research Center, National Institute on Drug Abuse, Baltimore, MD


    Stock #: JFS12890J

    ISSN: 0022-1198

    DOI: 10.1520/JFS12890J

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    Title Validity Testing of Commercial Urine Cocaine Metabolite Assays: IV. Evaluation of the EMIT® d.a.u.™ Cocaine Metabolite Assay in a Quantitative Mode for Detection of Cocaine Metabolite
    Symposium , 0000-00-00
    Committee E30