Volume 35, Issue 3 (May 1990)

    Hae III—A Suitable Restriction Endonuclease for Restriction Fragment Length Polymorphism Analysis of Biological Evidence Samples

    (Received 17 May 1989; accepted 19 June 1989)

    CODEN: JFSOAD

      Format Pages Price  
    PDF 7 $25   ADD TO CART


    Abstract

    Hae III has been selected by our laboratories as the restriction endonuclease of choice for restriction fragment length polymorphism analysis of forensic science samples. The enzyme is compatible with the D2S44 probe system and generates relatively small DNA fragments for that marker system. Similarly, Hae III is compatible with several other independent polymorphic loci, including D1S7, D4S139, D16S85, D17S74, D17S79, D14S13, and D20S15. Hae III is functional under a variety of adverse conditions for DNA digestion and is not affected by the methylation pattern in mammals. Finally, Hae III is a relatively inexpensive restriction endonuclease.


    Author Information:

    Budowle, B
    Research chemists, Forensic Science Research and Training Center, FBI Academy, Quantico, VA

    Samuel Baechtel, F
    Research chemists, Forensic Science Research and Training Center, FBI Academy, Quantico, VA

    Shutler, GG
    Molecular genetics specialists, Royal Canadian Mounted Police, Central Forensic Laboratory, Ottawa, ON

    Waye, JS
    Molecular genetics specialists, Royal Canadian Mounted Police, Central Forensic Laboratory, Ottawa, ON


    Stock #: JFS12858J

    ISSN: 0022-1198

    DOI: 10.1520/JFS12858J

    ASTM International
    is a member of CrossRef.

    Author
    Title Hae III—A Suitable Restriction Endonuclease for Restriction Fragment Length Polymorphism Analysis of Biological Evidence Samples
    Symposium , 0000-00-00
    Committee E30