ISSN: 0022-1198
CODEN: JFSCA
Page Count: 7

Hae III—A Suitable Restriction Endonuclease for Restriction Fragment Length Polymorphism Analysis of Biological Evidence Samples
Budowle, B
Research chemists, Forensic Science Research and Training Center, FBI Academy, VA

Samuel Baechtel, F
Research chemists, Forensic Science Research and Training Center, FBI Academy, VA

Shutler, GG
Molecular genetics specialists, Royal Canadian Mounted Police, Central Forensic Laboratory, ON

Waye, JS
Molecular genetics specialists, Royal Canadian Mounted Police, Central Forensic Laboratory, ON

(Received 17 May 1989; accepted 19 June 1989)

Abstract

Hae III has been selected by our laboratories as the restriction endonuclease of choice for restriction fragment length polymorphism analysis of forensic science samples. The enzyme is compatible with the D2S44 probe system and generates relatively small DNA fragments for that marker system. Similarly, Hae III is compatible with several other independent polymorphic loci, including D1S7, D4S139, D16S85, D17S74, D17S79, D14S13, and D20S15. Hae III is functional under a variety of adverse conditions for DNA digestion and is not affected by the methylation pattern in mammals. Finally, Hae III is a relatively inexpensive restriction endonuclease.

Keywords:
biology and pathology, deoxyribonucleic acid (DNA), enzymes, restriction fragment length polymorphism, restriction endonuclease, Hae III, D2S44 (YNH24), methylation, variable number of tandem repeats

Paper ID: JFS12858J
DOI: 10.1520/JFS12858J
ASTM International is a member of CrossRef.