ISSN: 0022-1198
CODEN: JFSCA
Page Count: 3

An Improved Method for Complement Subcomponent C1R Typing
Kamboh, MI
Assistant professor and professor, Graduate School of Public Health, University of Pittsburgh, PA

Ferrell, RE
Assistant professor and professor, Graduate School of Public Health, University of Pittsburgh, PA

(Received 6 September 1988; accepted 27 January 1989)

Abstract

An improved method has been developed for the reliable classification of different C1R genetic variant forms from human serum or plasma. The method combines the use of neuraminidase-digested samples followed by monodimensional isoelectric focusing in the pH range 5 to 8 followed by immunoblotting. The method yields a simple pattern, with one major band in homozygote and two major bands in heterozygote cases.

Keywords:
forensic science, genetic typing, isoelectric focusing, immunoblotting, C1R, genetic variants, forensic serology, neuraminidase, blood samples

Paper ID: JFS12817J
DOI: 10.1520/JFS12817J
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