ISSN: 0022-1198
CODEN: JFSCA
Page Count: 16

Evaluation of a Nonequilibrium Isoelectric Focusing (IEF) Method for the Simultaneous Typing of Esterase D (EsD), Red Cell Acid Phosphatase (AcP¹), Phosphoglucomutase (PGM¹), Adenylate Kinase (AK), and Adenosine Deaminase (ADA)
Stockwell, DC
Criminalists, San Bernardino County Sheriff's Regional Forensic Science Laboratory, CA

Gregonis, DJ
Criminalists, San Bernardino County Sheriff's Regional Forensic Science Laboratory, CA

Jones, DT
Criminalists, San Bernardino County Sheriff's Regional Forensic Science Laboratory, CA

(Received 22 December 1988; accepted 28 February 1989)

Abstract

A nonequilibrium isoelectric focusing method incorporating the chemical spacers MOPS and HEPES was developed and subsequently evaluated for its ability to reliably discriminate common and rare phenotypes in the esterase D (EsD), red cell acid phosphatase (AcP¹), phosphoglucomutase (PGM¹), adenylate kinase (AK), and adenosine deaminase (ADA) isoenzyme systems. The validation procedures used were blind testing, comparison of results to conventional methods, and evaluation of known rare variant phenotypes. This method proved to be a quick and reliable method for typing all five isoenzyme systems, while providing an excellent probability of discrimination (PD = 0.96).

Keywords:
forensic science, genetic typing, isoelectric focusing, nonequilibrium isoelectric focusing, chemical spacers, separators, ultrathin polyacrylamide gel, esterase D, red cell acid phosphatase, phosphoglucomutase, adenylate kinase, adenosine deaminase, rare phenotypes

Paper ID: JFS12800J
DOI: 10.1520/JFS12800J
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