Journal Published Online: 01 January 1990
Volume 35, Issue 1

Evaluation of a Nonequilibrium Isoelectric Focusing (IEF) Method for the Simultaneous Typing of Esterase D (EsD), Red Cell Acid Phosphatase (AcP ), Phosphoglucomutase (PGM ), Adenylate Kinase (AK), and Adenosine Deaminase (ADA)

CODEN: JFSCAS

Abstract

A nonequilibrium isoelectric focusing method incorporating the chemical spacers MOPS and HEPES was developed and subsequently evaluated for its ability to reliably discriminate common and rare phenotypes in the esterase D (EsD), red cell acid phosphatase (AcP1), phosphoglucomutase (PGM1), adenylate kinase (AK), and adenosine deaminase (ADA) isoenzyme systems. The validation procedures used were blind testing, comparison of results to conventional methods, and evaluation of known rare variant phenotypes. This method proved to be a quick and reliable method for typing all five isoenzyme systems, while providing an excellent probability of discrimination (PD = 0.96).

Author Information

Stockwell, DC
San Bernardino County Sheriff's Regional Forensic Science Laboratory, San Bernardino, CA
Gregonis, DJ
San Bernardino County Sheriff's Regional Forensic Science Laboratory, San Bernardino, CA
Jones, DT
San Bernardino County Sheriff's Regional Forensic Science Laboratory, San Bernardino, CA
Pages: 16
Price: $25.00
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Stock #: JFS12800J
ISSN: 0022-1198
DOI: 10.1520/JFS12800J