Volume 35, Issue 1 (January 1990)

    Evaluation of a Nonequilibrium Isoelectric Focusing (IEF) Method for the Simultaneous Typing of Esterase D (EsD), Red Cell Acid Phosphatase (AcP1), Phosphoglucomutase (PGM1), Adenylate Kinase (AK), and Adenosine Deaminase (ADA)

    (Received 22 December 1988; accepted 28 February 1989)

    CODEN: JFSOAD

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    Abstract

    A nonequilibrium isoelectric focusing method incorporating the chemical spacers MOPS and HEPES was developed and subsequently evaluated for its ability to reliably discriminate common and rare phenotypes in the esterase D (EsD), red cell acid phosphatase (AcP1), phosphoglucomutase (PGM1), adenylate kinase (AK), and adenosine deaminase (ADA) isoenzyme systems. The validation procedures used were blind testing, comparison of results to conventional methods, and evaluation of known rare variant phenotypes. This method proved to be a quick and reliable method for typing all five isoenzyme systems, while providing an excellent probability of discrimination (PD = 0.96).


    Author Information:

    Stockwell, DC
    Criminalists, San Bernardino County Sheriff's Regional Forensic Science Laboratory, San Bernardino, CA

    Gregonis, DJ
    Criminalists, San Bernardino County Sheriff's Regional Forensic Science Laboratory, San Bernardino, CA

    Jones, DT
    Criminalists, San Bernardino County Sheriff's Regional Forensic Science Laboratory, San Bernardino, CA


    Stock #: JFS12800J

    ISSN: 0022-1198

    DOI: 10.1520/JFS12800J

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    Title Evaluation of a Nonequilibrium Isoelectric Focusing (IEF) Method for the Simultaneous Typing of Esterase D (EsD), Red Cell Acid Phosphatase (AcP1), Phosphoglucomutase (PGM1), Adenylate Kinase (AK), and Adenosine Deaminase (ADA)
    Symposium , 0000-00-00
    Committee E30