Behavior of Animal Blood in Blood Typing Systems. Isoelectric Focusing of Erythrocyte Acid Phosphatase and Phosphoglucomutase

    Volume 34, Issue 5 (September 1989)

    ISSN: 0022-1198

    CODEN: JFSOAD

    Page Count: 9


    Thomson, DG
    Forensic biologist, technician, and forensic biologists, Criminalistics and Biology Section, Chemistry Division. Department of Scientific and Industrial Research, Petone,

    Dick, GL
    Forensic biologist, technician, and forensic biologists, Criminalistics and Biology Section, Chemistry Division. Department of Scientific and Industrial Research, Petone,

    Stowell, LI
    Forensic biologist, technician, and forensic biologists, Criminalistics and Biology Section, Chemistry Division. Department of Scientific and Industrial Research, Petone,

    Vintiner, SK
    Forensic biologist, technician, and forensic biologists, Criminalistics and Biology Section, Chemistry Division. Department of Scientific and Industrial Research, Petone,

    (Received 26 October 1988; accepted 23 November 1988)

    Abstract

    Isoenzyme band patterns of animal blood erythrocyte acid phosphatase (EAP) and phosphoglucomutase-1 (PGM) were studied by isoelectric focusing on ultrathin polyacrylamide gels. For blood from all animals tested (dog, cat, cow, sheep, and goat), the overall band patterns for both isoenzymes were different from those of the most common human types of these enzymes, although some animal EAP and PGM bands appeared in the human band areas. When mixtures of human and animal red blood cells were studied, it was found that misinterpretation of human types was possible only if the overall band pattern of the mixtures was ignored. For the animal blood tested, the strong PGM bands appearing outside the human band areas could be used as “markers” for the possible presence of animal blood in the samples tested.


    Paper ID: JFS12746J

    DOI: 10.1520/JFS12746J

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    Title Behavior of Animal Blood in Blood Typing Systems. Isoelectric Focusing of Erythrocyte Acid Phosphatase and Phosphoglucomutase
    Symposium , 0000-00-00
    Committee E30