ISSN: 0022-1198
CODEN: JFSCA
Page Count: 8

The Detection of Soluble ABH Blood Group Substances in Semen and Saliva Using Monoclonal Blood Grouping Reagents
Mudd, JL
Research chemist, FBI Academy, Forensic Science Research and Training Center, VA

(Received 23 July 1988; accepted 16 November 1988)

Abstract

Using an enzyme-linked immunosorbent assay (ELISA), this study investigated the use of monoclonal antibodies for detecting secreted ABH blood group substances in semen and saliva. The results demonstrated that the behavior of some monoclonals were unpredictable and often failed to detect the corresponding antigen in a number of the specimens tested. The suitability of the monoclonal reagents for detecting soluble blood group antigens could not be predicted by their behavior with red cell antigens. Consequently, care must be taken in the selection of monoclonal reagents for use in the detection of secreted blood group antigens.

Keywords:
forensic science, genetic typing, semen, saliva, blood groups, ABO blood group system, monoclonal antibodies, ELISA, immunoassay

Paper ID: JFS12744J
DOI: 10.1520/JFS12744J
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