Sex Determination of Bloodstains by the Use of a Ribonucleic Acid (RNA) Probe

    Volume 34, Issue 5 (September 1989)

    ISSN: 0022-1198

    CODEN: JFSOAD

    Page Count: 4


    Kobayashi, R
    Forensic scientist and professor, Juntendo University School of Medicine, Tokyo,

    Matsuzawa, S
    Forensic scientist and professor, Juntendo University School of Medicine, Tokyo,

    (Received 3 August 1988; accepted 20 December 1988)

    Abstract

    An asymmetric ribonucleic acid (RNA) probe, which represents a Y-chromosome specific nucleotide sequence, was applied to the sex origin of bloodstains by using a nonisotopic Southern hybridization method. The RNA probe was in vitro transcribed from the recombinant of the Y-chromosome specific deoxyribonucleic acid (DNA) sequence and transcription-vector Bluescript SK M13+. The use of this RNA probe can lower the detection limit of the nonisotopic DNA analyzing method to ⅛ of that reached by our previously reported method in which a DNA probe was used.


    Paper ID: JFS12743J

    DOI: 10.1520/JFS12743J

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    Title Sex Determination of Bloodstains by the Use of a Ribonucleic Acid (RNA) Probe
    Symposium , 0000-00-00
    Committee E30