ISSN: 0022-1198
CODEN: JFSCA
Page Count: 4

Sex Determination of Bloodstains by the Use of a Ribonucleic Acid (RNA) Probe
Kobayashi, R
Forensic scientist and professor, Juntendo University School of Medicine,

Matsuzawa, S
Forensic scientist and professor, Juntendo University School of Medicine,

(Received 3 August 1988; accepted 20 December 1988)

Abstract

An asymmetric ribonucleic acid (RNA) probe, which represents a Y-chromosome specific nucleotide sequence, was applied to the sex origin of bloodstains by using a nonisotopic Southern hybridization method. The RNA probe was in vitro transcribed from the recombinant of the Y-chromosome specific deoxyribonucleic acid (DNA) sequence and transcription-vector Bluescript SK M13⁺. The use of this RNA probe can lower the detection limit of the nonisotopic DNA analyzing method to ⅛ of that reached by our previously reported method in which a DNA probe was used.

Keywords:
forensic science, ribonucleic acid, blood, human identification

Paper ID: JFS12743J
DOI: 10.1520/JFS12743J
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