Volume 34, Issue 5 (September 1989)

    The Effects of Environment and Substrata on Deoxyribonucleic Acid (DNA): The Use of Casework Samples from New York City

    (Received 5 May 1988; accepted 18 November 1988)

    CODEN: JFSOAD

      Format Pages Price  
    PDF Version 8 $25   ADD TO CART


    Abstract

    This study was designed to analyze the effects of the environment and substrata on the quality of deoxyribonucleic acid (DNA) isolated from evidentiary specimens. The quality of DNA isolated from actual casework specimens was determined by measuring its size by agarose gel electrophoresis. The information obtained could be used to predict the suitability of the DNA in the samples for restriction fragment length polymorphism (RFLP) analysis. The evidentiary specimens chosen for DNA were classified according to substrate (scrapings, plastic bags, synthetics, denim, and carpet) and according to a subjective evaluation of the condition of the stain (soiled, damp, or putrified) and to its size (small or large). The results show that DNA of sufficient quality and high molecular weight (HMW) can be reliably isolated from bloodstains deposited on evidentiary items which have an unknown environmental history and which have dried onto a variety of substrata. Subsequent RFLP analysis of a selected number of these samples verified that the DNA was suitable for this type of analysis.


    Author Information:

    Balazs, I
    Forensic scientist, director of forensic science, manager of forensic and paternity laboratory, and director of laboratories, Lifecodes Corporation, Valhalla, NY

    Kobilinsky, L
    Professor of biology and immunology, and professor of criminalistics, John Jay College of Criminal Justice, City University of New York, New York, NY

    McNally, L
    Forensic scientist, director of forensic science, manager of forensic and paternity laboratory, and director of laboratories, Lifecodes Corporation, Valhalla, NY

    Baird, M
    Forensic scientist, director of forensic science, manager of forensic and paternity laboratory, and director of laboratories, Lifecodes Corporation, Valhalla, NY

    De Forest, P
    Professor of biology and immunology, and professor of criminalistics, John Jay College of Criminal Justice, City University of New York, New York, NY

    Shaler, RC
    Forensic scientist, director of forensic science, manager of forensic and paternity laboratory, and director of laboratories, Lifecodes Corporation, Valhalla, NY


    Stock #: JFS12742J

    ISSN: 0022-1198

    DOI: 10.1520/JFS12742J

    ASTM International
    is a member of CrossRef.

    Author
    Title The Effects of Environment and Substrata on Deoxyribonucleic Acid (DNA): The Use of Casework Samples from New York City
    Symposium , 0000-00-00
    Committee E30