ISSN: 0022-1198
CODEN: JFSCA
Page Count: 6

Assay of Free and Total Tocainide by High Performance Liquid Chromatography (HPLC) with Ultraviolet (UV) Detection
Guerra, C
Research assistant and professor, University of Texas Health Science Center, TZ

Wallace, JE
Research assistant and professor, University of Texas Health Science Center, TZ

Harris, SC
chief of staff, Veterans Administration Medical Center, MI

(Received 1 August 1988; accepted 11 October 1988)

Abstract

A rapid high performance liquid chromatographic (HPLC) method for the determination of tocainide, using N-(2,6-dimethylphenyl)-2-amino-butanimide as an internal standard, was developed. A methylene chloride extraction involving salting out at pH 9.0 was employed. An 85:15 mixture of 0.025M monobasic potassium phosphate at pH 3.0 and acetonitrile was used as the mobile phase. The separation and quantitative analysis of tocainide was performed on a mixed phase column with a 1.0-mL/min flow rate and detection at 210 nm. Separation of tocainide from some of its metabolites required the use of heptane sulfonic acid as an ionpairing reagent. For the free-drug assay, the specimen was centrifuged through an Amicon Centrifree filter before being processed.

Keywords:
toxicology, tocainide, chromatographic analysis, high performance liquid chromatography

Paper ID: JFS12720J
DOI: 10.1520/JFS12720J
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