Volume 34, Issue 3 (May 1989)

    Development and Application of Immunoassay for Paraquat: Radioimmunoassay

    (Received 1 July 1988; accepted 29 August 1988)

    CODEN: JFSOAD

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    Abstract

    A sensitive radioimmunoassay for paraquat is reported. Anti-paraquat antisera were produced by repeated immunization in rabbits with 1-methyl,1′-hexanoic acid-4,4′-bipyridinium (MHBP) coupled to bovine serum albumin (BSA). Less than 0.5 ng of paraquat dichloride was detectable by this assay system. These antisera were strongly cross-reactive with the bipyridyl ring and methyl group in either the 1- or 1′-position of paraquat. The determination of paraquat in tissues of paraquat-poisoned cadavers was also carried out.


    Author Information:

    Wu, B
    Postdoctoral student, professor, associate professor, and postdoctoral student, Hokkaido University School of Medicine, Sapporo,

    Wakasugi, C
    Professor, instructor, and instructor, Wakayama Medical College, Wakayama,

    Ikeda, H
    Professor, instructor, and instructor, Wakayama Medical College, Wakayama,

    Terazawa, K
    Postdoctoral student, professor, associate professor, and postdoctoral student, Hokkaido University School of Medicine, Sapporo,

    Masui, M
    Professor, instructor, and instructor, Wakayama Medical College, Wakayama,

    Nagao, M
    Postdoctoral student, professor, associate professor, and postdoctoral student, Hokkaido University School of Medicine, Sapporo,

    Takatori, T
    Postdoctoral student, professor, associate professor, and postdoctoral student, Hokkaido University School of Medicine, Sapporo,


    Stock #: JFS12675J

    ISSN: 0022-1198

    DOI: 10.1520/JFS12675J

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    Author
    Title Development and Application of Immunoassay for Paraquat: Radioimmunoassay
    Symposium , 0000-00-00
    Committee E30