ISSN: 0022-1198
CODEN: JFSCA
Page Count: 6

Development and Application of Immunoassay for Paraquat: Radioimmunoassay
Wu, B
Postdoctoral student, professor, associate professor, and postdoctoral student, Hokkaido University School of Medicine,

Wakasugi, C
Professor, instructor, and instructor, Wakayama Medical College,

Ikeda, H
Professor, instructor, and instructor, Wakayama Medical College,

Terazawa, K
Postdoctoral student, professor, associate professor, and postdoctoral student, Hokkaido University School of Medicine,

Masui, M
Professor, instructor, and instructor, Wakayama Medical College,

Nagao, M
Postdoctoral student, professor, associate professor, and postdoctoral student, Hokkaido University School of Medicine,

Takatori, T
Postdoctoral student, professor, associate professor, and postdoctoral student, Hokkaido University School of Medicine,

(Received 1 July 1988; accepted 29 August 1988)

Abstract

A sensitive radioimmunoassay for paraquat is reported. Anti-paraquat antisera were produced by repeated immunization in rabbits with 1-methyl,1′-hexanoic acid-4,4′-bipyridinium (MHBP) coupled to bovine serum albumin (BSA). Less than 0.5 ng of paraquat dichloride was detectable by this assay system. These antisera were strongly cross-reactive with the bipyridyl ring and methyl group in either the 1- or 1′-position of paraquat. The determination of paraquat in tissues of paraquat-poisoned cadavers was also carried out.

Keywords:
toxicology, paraquat, radioimmunoassay, tissues (biology)

Paper ID: JFS12675J
DOI: 10.1520/JFS12675J
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