Volume 34, Issue 2 (March 1989)

    A Study of Sex Identification of Trace, Dried Bloodstains Using a Y-Chromosome-Specific Deoxyribonucleic Acid (DNA) Probe

    (Received 25 March 1988; accepted 22 June 1988)

    CODEN: JFSOAD

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    Abstract

    A new method is discussed which examines trace, dried bloodstains by gel in situ hybridization using a Y-chromosome-specific deoxyribonucleic acid (DNA) probe to determine the sex of the bloodstain for forensic medicine application. The complete DNA is transferred directly by electrophoresis onto the gel intact, bypassing the possibilities of impurities contaminating the sample and of DNA degradation. The method has proven accurate for small (2.5-mm-diameter) samples aged up to eight years and is quick, simple, and easily read.


    Author Information:

    Wang, G-L
    Chief legal medical expert, legal medical expert, assistant chief legal medical expert, chief legal medical expert of examination, and assistant chief legal medical expert, Liaoning Criminal Scientific and Technical Research Institute, Shenyang, Liaoning

    Wu, G-Y
    Assistant professor, technician, assistant lecturer, lecturer, and professor, Basic Medicine Research Institute, Chinese Academy of Medical Science, Beijing,

    Liu, J-Z
    Assistant professor, technician, assistant lecturer, lecturer, and professor, Basic Medicine Research Institute, Chinese Academy of Medical Science, Beijing,

    Gao, Q-S
    Assistant professor, technician, assistant lecturer, lecturer, and professor, Basic Medicine Research Institute, Chinese Academy of Medical Science, Beijing,

    Lu, S-H
    Chief legal medical expert, legal medical expert, assistant chief legal medical expert, chief legal medical expert of examination, and assistant chief legal medical expert, Liaoning Criminal Scientific and Technical Research Institute, Shenyang, Liaoning

    Wang, S-W
    Assistant professor, technician, assistant lecturer, lecturer, and professor, Basic Medicine Research Institute, Chinese Academy of Medical Science, Beijing,

    Jiang, X-H
    Chief legal medical expert, legal medical expert, assistant chief legal medical expert, chief legal medical expert of examination, and assistant chief legal medical expert, Liaoning Criminal Scientific and Technical Research Institute, Shenyang, Liaoning

    Zhu, Y-W
    Chief legal medical expert, legal medical expert, assistant chief legal medical expert, chief legal medical expert of examination, and assistant chief legal medical expert, Liaoning Criminal Scientific and Technical Research Institute, Shenyang, Liaoning

    Shen, Y
    Assistant professor, technician, assistant lecturer, lecturer, and professor, Basic Medicine Research Institute, Chinese Academy of Medical Science, Beijing,

    He, Z-N
    Chief legal medical expert, legal medical expert, assistant chief legal medical expert, chief legal medical expert of examination, and assistant chief legal medical expert, Liaoning Criminal Scientific and Technical Research Institute, Shenyang, Liaoning


    Stock #: JFS12643J

    ISSN: 0022-1198

    DOI: 10.1520/JFS12643J

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    Author
    Title A Study of Sex Identification of Trace, Dried Bloodstains Using a Y-Chromosome-Specific Deoxyribonucleic Acid (DNA) Probe
    Symposium , 0000-00-00
    Committee E30