ISSN: 0022-1198
CODEN: JFSCA
Page Count: 6

A Study of Sex Identification of Trace, Dried Bloodstains Using a Y-Chromosome-Specific Deoxyribonucleic Acid (DNA) Probe
Wang, G-L
Chief legal medical expert, legal medical expert, assistant chief legal medical expert, chief legal medical expert of examination, and assistant chief legal medical expert, Liaoning Criminal Scientific and Technical Research Institute, Liaoning

Wu, G-Y
Assistant professor, technician, assistant lecturer, lecturer, and professor, Basic Medicine Research Institute, Chinese Academy of Medical Science,

Liu, J-Z
Assistant professor, technician, assistant lecturer, lecturer, and professor, Basic Medicine Research Institute, Chinese Academy of Medical Science,

Gao, Q-S
Assistant professor, technician, assistant lecturer, lecturer, and professor, Basic Medicine Research Institute, Chinese Academy of Medical Science,

Lu, S-H
Chief legal medical expert, legal medical expert, assistant chief legal medical expert, chief legal medical expert of examination, and assistant chief legal medical expert, Liaoning Criminal Scientific and Technical Research Institute, Liaoning

Wang, S-W
Assistant professor, technician, assistant lecturer, lecturer, and professor, Basic Medicine Research Institute, Chinese Academy of Medical Science,

Jiang, X-H
Chief legal medical expert, legal medical expert, assistant chief legal medical expert, chief legal medical expert of examination, and assistant chief legal medical expert, Liaoning Criminal Scientific and Technical Research Institute, Liaoning

Zhu, Y-W
Chief legal medical expert, legal medical expert, assistant chief legal medical expert, chief legal medical expert of examination, and assistant chief legal medical expert, Liaoning Criminal Scientific and Technical Research Institute, Liaoning

Shen, Y
Assistant professor, technician, assistant lecturer, lecturer, and professor, Basic Medicine Research Institute, Chinese Academy of Medical Science,

He, Z-N
Chief legal medical expert, legal medical expert, assistant chief legal medical expert, chief legal medical expert of examination, and assistant chief legal medical expert, Liaoning Criminal Scientific and Technical Research Institute, Liaoning

(Received 25 March 1988; accepted 22 June 1988)

Abstract

A new method is discussed which examines trace, dried bloodstains by gel in situ hybridization using a Y-chromosome-specific deoxyribonucleic acid (DNA) probe to determine the sex of the bloodstain for forensic medicine application. The complete DNA is transferred directly by electrophoresis onto the gel intact, bypassing the possibilities of impurities contaminating the sample and of DNA degradation. The method has proven accurate for small (2.5-mm-diameter) samples aged up to eight years and is quick, simple, and easily read.

Keywords:
forensic science, deoxyribonucleic acid, (DNA), electrophoresis, human identification, in situ hybridization, Y-chromosome, sex identification, bloodstains, agrose gel, extraction, fluorescence, ultraviolet

Paper ID: JFS12643J
DOI: 10.1520/JFS12643J
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