(Received 22 July 1987; accepted 15 March 1988)
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Three strains of Cunninghamella elegance (C. elegance), Penicillium simplicissimum (P. simpl.), and Aspergillus niger (A. niger) were isolated from soil that produced the enzymes acting on blood groups A and B red cells. Culture filtrates from these fungi contained α-N-acetyl-D-galactosaminidase as an A-decomposing enzyme, which resulted in an almost complete loss of A specificity and an enhancement of blood group H activity as measured by elution tests using monoclonal antibodies. They also contained an α-D-galactosidase and an α-L-fucosidase, which partially destroyed the blood group B specific activity, but did not influence the blood group H specific activity.
Graduate student of medicine, Nagasaki University School of Medicine, Nagasaki,
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