Validity Testing of Commercial Urine Cocaine Metabolite Assays: I. Assay Detection Times, Individual Excretion Patterns, and Kinetics After Cocaine Administration to Humans

    Volume 34, Issue 1 (January 1989)

    ISSN: 0022-1198

    CODEN: JFSOAD

    Page Count: 17


    Paul, BD
    Chief, Methods Development, commanding officer, and laboratory director, Navy Drug Screening Laboratory, Naval Air Station, Norfolk, VA

    Cone, EJ
    Chief and medical technologist (ASCP), Laboratory of Chemistry and Drug Metabolism, Addiction Research Center, National Institute on Drug Abuse, Baltimore, MD

    Mitchell, J
    Chief, Methods Development, commanding officer, and laboratory director, Navy Drug Screening Laboratory, Naval Air Station, Norfolk, VA

    Mell, LD
    Chief, Methods Development, commanding officer, and laboratory director, Navy Drug Screening Laboratory, Naval Air Station, Norfolk, VA

    Menchen, SL
    Chief and medical technologist (ASCP), Laboratory of Chemistry and Drug Metabolism, Addiction Research Center, National Institute on Drug Abuse, Baltimore, MD

    (Received 28 January 1988; accepted 13 May 1988)

    Abstract

    A validity study of eight commercial urine assays for detection of cocaine metabolite was performed on clinical specimens collected from human subjects who received single 20-mg intravenous doses of cocaine hydrochloride. The specimens were collected under controlled conditions and analyzed in random order under blind conditions. Benzoylecgonine concentration in each specimen also was determined by gas chromatography/mass spectrometry (GC/MS). Mean times of detection of the last positive specimen (≥ 300 ng/mL of benzoylecgonine equivalents) after cocaine administration varied among seven of the commercial tests from 16.9 to 52.9 h in the following aseending order: Toxi-Lab® < TDx® = EMIT® dau™ = EMIT® st™ < Abuscreen® < Coat-A-Count® = Double Antibody. In contrast, a commercial spot test (KDI Quik Test™) which was evaluated for detection of cocaine metabolite produced both false positives and false negatives for benzoylecgonine and was not considered to be a valid test for detection of cocaine metabolite. Half-lives of excretion of benzoylecgonine among four subjects varied from 5.9 to 7.9 h, and overall recovery of benzoylecgonine varied from 15.0 to 34.3% of the administered dose of cocaine.


    Paper ID: JFS12607J

    DOI: 10.1520/JFS12607J

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    Title Validity Testing of Commercial Urine Cocaine Metabolite Assays: I. Assay Detection Times, Individual Excretion Patterns, and Kinetics After Cocaine Administration to Humans
    Symposium , 0000-00-00
    Committee E30