ISSN: 0022-1198
CODEN: JFSCA
Page Count: 6

Group Specific Component Subtyping in Bloodstains by Separator Isoelectric Focusing in Micro-Ultrathin Polyacrylamide Gels Followed by Immunoblotting
Alonso, A
Professor, Biology Section, National Institute of Toxicology,

(Received 21 August 1987; accepted 20 January 1988)

Abstract

The identification of group specific component (Gc) subtypes derived from bloodstains by separator isoelectric focusing in micro-ultrathin polyacrylamide gels (interelectrode distance: 50 mm) containing 4.5 to 5.4 pharmalytes is described. The separation achieved between Gc 1F and Gc 1S bands is compared favorably with that obtained using separator isoelectric focusing in conventional polyacrylamide gels dimensions (interelectrode distance: 110 to 120 mm). The technique is rapid and economical, and the immunoblotting method described is more sensitive than immunofixation followed by silver staining.

Keywords:
pathology and biology, group specific component, genetic typing, isoelectric focusing, bloodstains, micro-ultrathin polyacrylamide gel, immunoblotting

Paper ID: JFS12563J
DOI: 10.1520/JFS12563J
ASTM International is a member of CrossRef.