Group Specific Component Subtyping in Bloodstains by Separator Isoelectric Focusing in Micro-Ultrathin Polyacrylamide Gels Followed by Immunoblotting

    Volume 33, Issue 5 (September 1988)

    ISSN: 0022-1198

    CODEN: JFSOAD

    Page Count: 6


    Alonso, A
    Professor, Biology Section, National Institute of Toxicology, Madrid,

    (Received 21 August 1987; accepted 20 January 1988)

    Abstract

    The identification of group specific component (Gc) subtypes derived from bloodstains by separator isoelectric focusing in micro-ultrathin polyacrylamide gels (interelectrode distance: 50 mm) containing 4.5 to 5.4 pharmalytes is described. The separation achieved between Gc 1F and Gc 1S bands is compared favorably with that obtained using separator isoelectric focusing in conventional polyacrylamide gels dimensions (interelectrode distance: 110 to 120 mm). The technique is rapid and economical, and the immunoblotting method described is more sensitive than immunofixation followed by silver staining.


    Paper ID: JFS12563J

    DOI: 10.1520/JFS12563J

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    Title Group Specific Component Subtyping in Bloodstains by Separator Isoelectric Focusing in Micro-Ultrathin Polyacrylamide Gels Followed by Immunoblotting
    Symposium , 0000-00-00
    Committee E30