Journal Published Online: 01 September 1988
Volume 33, Issue 5

Rapid Isolation of Benzodiazepines with Sep-Pak® C Cartridges

CODEN: JFSCAS

Abstract

A rapid method for isolation of various benzodiazepines from human samples with Sep-Pak® C18 cartridges before wide-bore capillary gas chromatography is described. The drugs dissolved in alkaline samples were directly applied to the cartridges and eluted with hexane/isopropanol (9:1). The recoveries were excellent for all drugs in urine samples, but were somewhat lower for some drugs in plasma samples. The latter problem could be easily circumvented by using a deproteinization process before their application to the cartridge. We can recommend the Sep-Pak C18 cartridges for isolation of benzodiazepines because of their simplicity and rapidity.

Author Information

Suzuki, O
Hamamatsu University School of Medicine, Hamamatsu, Japan
Seno, H
Hamamatsu University School of Medicine, Hamamatsu, Japan
Kumazawa, T
Hamamatsu University School of Medicine, Hamamatsu, Japan
Pages: 5
Price: $25.00
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Stock #: JFS12560J
ISSN: 0022-1198
DOI: 10.1520/JFS12560J