Rapid Isolation of Benzodiazepines with Sep-Pak® C18 Cartridges

    Volume 33, Issue 5 (September 1988)

    ISSN: 0022-1198

    CODEN: JFSOAD

    Page Count: 5


    Seno, H
    Associate professor, post-graduate student, and research associate, Hamamatsu University School of Medicine, Hamamatsu,

    Suzuki, O
    Associate professor, post-graduate student, and research associate, Hamamatsu University School of Medicine, Hamamatsu,

    Kumazawa, T
    Associate professor, post-graduate student, and research associate, Hamamatsu University School of Medicine, Hamamatsu,

    (Received 14 December 1987; accepted 11 January 1988)

    Abstract

    A rapid method for isolation of various benzodiazepines from human samples with Sep-Pak® C18 cartridges before wide-bore capillary gas chromatography is described. The drugs dissolved in alkaline samples were directly applied to the cartridges and eluted with hexane/isopropanol (9:1). The recoveries were excellent for all drugs in urine samples, but were somewhat lower for some drugs in plasma samples. The latter problem could be easily circumvented by using a deproteinization process before their application to the cartridge. We can recommend the Sep-Pak C18 cartridges for isolation of benzodiazepines because of their simplicity and rapidity.


    Paper ID: JFS12560J

    DOI: 10.1520/JFS12560J

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    Title Rapid Isolation of Benzodiazepines with Sep-Pak® C18 Cartridges
    Symposium , 0000-00-00
    Committee E30