ISSN: 0022-1198
CODEN: JFSCA
Page Count: 5

Rapid Isolation of Benzodiazepines with Sep-Pak® C¹⁸ Cartridges
Seno, H
Associate professor, post-graduate student, and research associate, Hamamatsu University School of Medicine,

Suzuki, O
Associate professor, post-graduate student, and research associate, Hamamatsu University School of Medicine,

Kumazawa, T
Associate professor, post-graduate student, and research associate, Hamamatsu University School of Medicine,

(Received 14 December 1987; accepted 11 January 1988)

Abstract

A rapid method for isolation of various benzodiazepines from human samples with Sep-Pak® C¹⁸ cartridges before wide-bore capillary gas chromatography is described. The drugs dissolved in alkaline samples were directly applied to the cartridges and eluted with hexane/isopropanol (9:1). The recoveries were excellent for all drugs in urine samples, but were somewhat lower for some drugs in plasma samples. The latter problem could be easily circumvented by using a deproteinization process before their application to the cartridge. We can recommend the Sep-Pak C¹⁸ cartridges for isolation of benzodiazepines because of their simplicity and rapidity.

Keywords:
toxicology, benzodiazepines, Sep-Pak® C18 cartridges, chromatographic analysis, wide-bore capillary gas chromatography

Paper ID: JFS12560J
DOI: 10.1520/JFS12560J
ASTM International is a member of CrossRef.