ISSN: 0022-1198
CODEN: JFSCA
Page Count: 8

Establishing Paternity Using Minisatellite DNA Probes When the Putative Father Is Unavailable for Testing
Hossaini, AA
Professor, Department of Pathology, VA

Demers, DB
Graduate student, Department of Pathology, and assistant professor, Hematology/Oncology, Medical College of Virginia, VA

Westin, EH
Graduate student, Department of Pathology, and assistant professor, Hematology/Oncology, Medical College of Virginia, VA

Odelberg, SJ
postdoctoral research associate, University of Utah, UT

(Received 15 July 1987; accepted 20 October 1987)

Abstract

A paternity case involving a putative father who had died a few years earlier in an automobile accident was referred to the laboratory for testing. The child and his mother, the deceased's parents, and nine of the deceased's siblings were available for analysis. As previously reported, paternity testing using red blood cell groups, human leukocyte antigens (HLA), red blood cell enzymes, serum proteins, and immunoglobulin allotypes gave a cumulative paternity index of 43 300 and a combined probability of paternity equal to 99.998%. RFLP analysis using Hinf 1 and Sau 3A single digests and the minisatellite deoxyribonucleic acid (DNA) probes 15.1.11.4 and 6.3 showed no exclusion of paternity and gave nearly conclusive evidence that the putative father was the biological father of the child.

Keywords:
pathology and biology, paternity, deoxyribonucleic acid (DNA), restriction fragment length polymorphism (RFLP)

Paper ID: JFS12515J
DOI: 10.1520/JFS12515J
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