An Improved Method for the Histological Preparation of Single Hairs and Dust Samples—Morphological and Immunological Examination

    Volume 33, Issue 3 (May 1988)

    ISSN: 0022-1198

    CODEN: JFSOAD

    Page Count: 6


    Ishiyama, I
    Postgraduate student, professor, and medical technician, Faculty of Medicine, University of Tokyo, Tokyo,

    Tanabe, R
    Postgraduate student, professor, and medical technician, Faculty of Medicine, University of Tokyo, Tokyo,

    Itakura, Y
    Postgraduate student, professor, and medical technician, Faculty of Medicine, University of Tokyo, Tokyo,

    (Received 16 July 1987; accepted 14 September 1987)

    Abstract

    An improved method of the serological and morphological investigation of human hair is reported. The hair was firmly fixed onto a microscopic slide with cellophane tape and observed microscopically to confirm the cuticula images and the presence of the medulla. A piece of the hair containing the medulla was dissected, embedded in paraffin, and a cross section of this hair was prepared. By treating the sample with immunohistochemistry (biotin-antibiotin ABC technique), the blood type of the hair was confirmed definitively. Dust containing shaved beard can be examined in the same way.


    Paper ID: JFS12487J

    DOI: 10.1520/JFS12487J

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    Title An Improved Method for the Histological Preparation of Single Hairs and Dust Samples—Morphological and Immunological Examination
    Symposium , 0000-00-00
    Committee E30