Volume 33, Issue 3 (May 1988)

    A Hybrid Ampholyte Focusing Technique for Esterase D Subtyping of Evidentiary Material

    (Received 22 June 1987; accepted 27 July 1987)

    CODEN: JFSOAD

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    Abstract

    An ultrathin-layer polyacrylamide gel isoelectric focusing technique that uses a composite of ampholytes from three commercial sources is described for subtyping esterase D. All common allelic products of esterase D were separated clearly. The technique described in this paper provides a higher conclusive call rate on known blood specimens (95.8%) and questioned bloodstains (69.7%) compared with continuous zone electrophoresis in agarose gels (89.9 and 37.6%, respectively).


    Author Information:

    Gambel, AM
    Research chemist and scientist, Forensic Science Research and Training Section, FBI Academy, Quantico, VA

    Budowle, B
    Research chemist and scientist, Forensic Science Research and Training Section, FBI Academy, Quantico, VA


    Stock #: JFS12482J

    ISSN: 0022-1198

    DOI: 10.1520/JFS12482J

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    Author
    Title A Hybrid Ampholyte Focusing Technique for Esterase D Subtyping of Evidentiary Material
    Symposium , 0000-00-00
    Committee E30