ISSN: 0022-1198
CODEN: JFSCA
Page Count: 6

A Hybrid Ampholyte Focusing Technique for Esterase D Subtyping of Evidentiary Material
Gambel, AM
Research chemist and scientist, Forensic Science Research and Training Section, FBI Academy, VA

Budowle, B
Research chemist and scientist, Forensic Science Research and Training Section, FBI Academy, VA

(Received 22 June 1987; accepted 27 July 1987)

Abstract

An ultrathin-layer polyacrylamide gel isoelectric focusing technique that uses a composite of ampholytes from three commercial sources is described for subtyping esterase D. All common allelic products of esterase D were separated clearly. The technique described in this paper provides a higher conclusive call rate on known blood specimens (95.8%) and questioned bloodstains (69.7%) compared with continuous zone electrophoresis in agarose gels (89.9 and 37.6%, respectively).

Keywords:
forensic science, esterase D, genetic typing, isoelectric focusing, bloodstains, ultrathin-layer polyacrylamide gel isoelectric focusing, agarose gel continuous zone electrophoresis, ampholytes, evidentiary material

Paper ID: JFS12482J
DOI: 10.1520/JFS12482J
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