ISSN: 0022-1198
CODEN: JFSCA
Page Count: 7

Identification of Male Bloodstains by Dot Hybridization of Human Y Chromosome-Specific Deoxyribonucleic Acid (DNA) Probe
Fukushima, H
Associate professor and professor, Shinshu University School of Medicine,

Hasekura, H
Associate professor and professor, Shinshu University School of Medicine,

Nagai, K
Senior lecturer, Tokyo Medical College,

(Received 28 April 1987; accepted 16 September 1987)

Abstract

The sex determination of bloodstains was performed using a human Y chromosome-specific (DNA) fragment of 1.9-kb length as a hybridization probe. The DNA samples were taken from 1- and 4-week-old bloodstains of males and females, respectively. Strong signals with male DNA were observed by Y-probe, while faint signals with female DNA were detected. In addition, clear signals were observed in the extract samples from male bloodstains (16-week-old) on paper. Dot hybridization of the Y-probe would be widely applicable to studies on sex determination of medicolegal materials such as blood, bloodstains, teeth, and cadaverous parts.

Keywords:
forensic science, human identification, blood, deoxyribonucleic acid (DNA), dot hybridization, bloodstains, Y chromosome-specific DNA probe

Paper ID: JFS12471J
DOI: 10.1520/JFS12471J
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