Volume 33, Issue 3 (May 1988)

    Identification of Male Bloodstains by Dot Hybridization of Human Y Chromosome-Specific Deoxyribonucleic Acid (DNA) Probe

    (Received 28 April 1987; accepted 16 September 1987)

    CODEN: JFSOAD

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    Abstract

    The sex determination of bloodstains was performed using a human Y chromosome-specific (DNA) fragment of 1.9-kb length as a hybridization probe. The DNA samples were taken from 1- and 4-week-old bloodstains of males and females, respectively. Strong signals with male DNA were observed by Y-probe, while faint signals with female DNA were detected. In addition, clear signals were observed in the extract samples from male bloodstains (16-week-old) on paper. Dot hybridization of the Y-probe would be widely applicable to studies on sex determination of medicolegal materials such as blood, bloodstains, teeth, and cadaverous parts.


    Author Information:

    Fukushima, H
    Associate professor and professor, Shinshu University School of Medicine, Nagano,

    Hasekura, H
    Associate professor and professor, Shinshu University School of Medicine, Nagano,

    Nagai, K
    Senior lecturer, Tokyo Medical College, Tokyo,


    Stock #: JFS12471J

    ISSN: 0022-1198

    DOI: 10.1520/JFS12471J

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    Title Identification of Male Bloodstains by Dot Hybridization of Human Y Chromosome-Specific Deoxyribonucleic Acid (DNA) Probe
    Symposium , 0000-00-00
    Committee E30