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    Volume 33, Issue 3 (May 1988)

    Sex Identification in Fresh Blood and Dried Bloodstains by a Nonisotopic Deoxyribonucleic Acid (DNA) Analyzing Technique

    (Received 20 March 1987; accepted 24 August 1987)

    CODEN: JFSOAD

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    Abstract

    Deoxyribonucleic acid (DNA) specimens were prepared from blood or bloodstain extracts, and the content of a Y-chromosome specific DNA fragment was investigated by the Southern hybridization method using a nonisotopic staining technique. Thus obtained patterns of male DNA showed a clear band, whereas broad stains with some faint bands appeared on the patterns of DNA from both sexes. This method is expected to be a new powerful mean of forensic medical examination.


    Author Information:

    Nakauchi, H
    Molecular biologist, Laboratory of Immunology, Juntendo University School of Medicine, Tokyo,

    Kobayashi, R
    Forensic scientist and professor, Juntendo University School of Medicine, Tokyo,

    Matsuzawa, S
    Forensic scientist and professor, Juntendo University School of Medicine, Tokyo,

    Nakagome, Y
    Geneticists, National Children's Medical Center, Tokyo,

    Nakahori, Y
    Geneticists, National Children's Medical Center, Tokyo,


    Stock #: JFS12470J

    ISSN: 0022-1198

    DOI: 10.1520/JFS12470J

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    Title Sex Identification in Fresh Blood and Dried Bloodstains by a Nonisotopic Deoxyribonucleic Acid (DNA) Analyzing Technique
    Symposium , 0000-00-00
    Committee E30