ISSN: 0022-1198
CODEN: JFSCA
Page Count: 8

Sex Identification in Fresh Blood and Dried Bloodstains by a Nonisotopic Deoxyribonucleic Acid (DNA) Analyzing Technique
Nakauchi, H
Molecular biologist, Laboratory of Immunology, Juntendo University School of Medicine,

Kobayashi, R
Forensic scientist and professor, Juntendo University School of Medicine,

Matsuzawa, S
Forensic scientist and professor, Juntendo University School of Medicine,

Nakagome, Y
Geneticists, National Children's Medical Center,

Nakahori, Y
Geneticists, National Children's Medical Center,

(Received 20 March 1987; accepted 24 August 1987)

Abstract

Deoxyribonucleic acid (DNA) specimens were prepared from blood or bloodstain extracts, and the content of a Y-chromosome specific DNA fragment was investigated by the Southern hybridization method using a nonisotopic staining technique. Thus obtained patterns of male DNA showed a clear band, whereas broad stains with some faint bands appeared on the patterns of DNA from both sexes. This method is expected to be a new powerful mean of forensic medical examination.

Keywords:
forensic science, deoxyribonucleic acid (DNA), blood, human identification

Paper ID: JFS12470J
DOI: 10.1520/JFS12470J
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