ISSN: 0022-1198
CODEN: JFSCA
Page Count: 7

Identification of Human Urinary Stains by Enzyme-Linked Immunosorbent Assay for Human Uromucoid
Okajima, H
Graduate student, assistant professor, and professor, Nagoya University School of Medicine,

Tsutsumi, H
Forensic chemist, Criminal Science Laboratory, Aichi Prefectural Police Headquarters,

Sato, K
Graduate student, assistant professor, and professor, Nagoya University School of Medicine,

Katsumata, Y
Graduate student, assistant professor, and professor, Nagoya University School of Medicine,

(Received 20 March 1987; accepted 20 April 1987)

Abstract

An enzyme-linked immunosorbent assay (ELISA) of the sandwich type for identification of human urinary stains using commercially available anti-human uromucoid was developed. When experimentally prepared urinary stains of humans and animals, 2 by 2 cm in area, were subjected to analysis, human stains could be differentiated from animal ones except chimpanzee and Old World monkey ones. Stains of other human body fluids showed negative reactions. The reactions did not decrease when human urinary stains were stored at room temperature for three months. The present ELISA provides a useful presumptive test for urinary stains of human origin.

Keywords:
pathology and biology, urine, immunoassay, proteins, uromucoid, urinary stains, enzyme-linked immunosorbent assay (ELISA)

Paper ID: JFS12456J
DOI: 10.1520/JFS12456J
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